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Showing: 8381 - 8400 of 9225 results

  2. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid
    #123363
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.
    Depositor
    Sidi Chen
    Article
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    EGFP-NLS
    Use
    CRISPR, Lentiviral, and Luciferase
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123363-plasmid-map-sequence-id-240469

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  5. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

    Plasmid
    #227333
    Purpose
    MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227333-plasmid-map-sequence-id-452137

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  7. TFORF2048

    Plasmid
    #143332
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRDM9 (PRDM9 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143332-plasmid-map-sequence-id-408544

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  8. pHtrA-TEV-His12

    Plasmid
    #137036
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137036-plasmid-map-sequence-id-275699

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  9. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid
    #135955
    Purpose
    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Article
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) N-terminal
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135955-plasmid-map-sequence-id-269512

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  10. pXPR_070

    Plasmid
    #164265
    Purpose
    Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164265-plasmid-map-sequence-id-330127

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  16. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

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Showing: 8381 - 8400 of 9225 results