Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 63,826 results for: cloning

Clear keywords
Showing: 8421 - 8440 of 63826 results

  1. AsRed2-N1

    Plasmid
    #54703
    Purpose
    Localization: N1 Cloning Vector, Excitation: 576, Emission: 592
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    AsRed2
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pUS250

    Plasmid
    #198322
    Purpose
    Cumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoprotein
    Depositor
    Nicholas Coleman, Mark Somerville
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    cumate-inducible
    Available since
    April 12, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    198322-plasmid-map-sequence-id-390064

    Enlarge

  3. pGEX6P1-DEST-Strep2

    Plasmid
    #119753
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Promoter
    tac promoter
    Available since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119753-plasmid-map-sequence-id-243311

    Enlarge

  6. pMA-SpCas9-g7

    Plasmid
    #80790
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80790-plasmid-map-sequence-id-161640

    Enlarge

  7. pMA-SpCas9-g6

    Plasmid
    #80789
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80789-plasmid-map-sequence-id-161638

    Enlarge

  13. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Publication
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210212-plasmid-map-sequence-id-416400

    Enlarge

  19. pICSL13008

    Plasmid
    #245656
    Purpose
    Level 0 Golden Gate part Long 35s gene cauliflower mosaic virus and 5 prime untranslated leader tobacco mosaic virus promoter, GGAG - CCAT
    Depositor
    Mark Youles
    Insert
    Long 35s gene cauliflower mosaic virus and 5 prime untranslated leader tobacco mosaic virus promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245656-plasmid-map-sequence-id-495550

    Enlarge

  20. pTRC-LIC

    Plasmid
    #62343
    Purpose
    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    tac promoter from pKK233-2
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62343-plasmid-map-sequence-id-102266

    Enlarge

Showing: 8421 - 8440 of 63826 results