We narrowed to 24,931 results for: Spr

1. pYPQ138A

   Plasmid

   #69280

   Purpose

   Golden Gate entry vector; 8th gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ137A

   Plasmid

   #69279

   Purpose

   Golden Gate entry vector;7th gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCD296.7

   Plasmid

   #113320

   Purpose

   dCas9, MCP-TetD, J107 scRNA.b1

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   dCas9, MCP-TetD, J107 scRNA.b1_MS2
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adra1b

   Plasmid

   #184293

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adra1b

   Depositor

   Bernardo Sabatini

   Inserts

   sgRNA-Adra1b
   sgRNA-Adra1b
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLS254

   Plasmid

   #73721

   Purpose

   SapTrap Halotag + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Halotag + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLS297

   Plasmid

   #73737

   Purpose

   SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   STOP codon + let-858 3'-UTR
   

   Use

   CRISPR

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJF121

   Plasmid

   #113325

   Purpose

   pTet-W108 scRNA.b2

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   W108 scRNA_MS2
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK504

   Plasmid

   #155381

   Purpose

   dCas12a crRNAs

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Insert

   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMLS269

   Plasmid

   #73727

   Purpose

   SapTrap SL2::SV40-NLS C-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   SL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)
   

   Use

   CRISPR

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_835

    Plasmid

    #245327

    Purpose

    Cas9 CRISPRko positive control guide; targets CD59

    Depositor

    John Doench, David Root

    Insert

    sgRNA CD59 (CD59 Synthetic)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. iC30 PB-Zim3-dCas9-P2A-BFP_hygro

    Plasmid

    #236743

    Purpose

    Constitutive expression of Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
    EF1a-hygro-WPRE
    

    Expression

    Mammalian

    Promoter

    CAG and EF1a

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. psLIB TRE mTFP1-actin

    Plasmid

    #229970

    Purpose

    encodes a mTFP1-actin fusion construct under the control of a dox inducible promoter

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    mTFP1-actin
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. psLIB TRE H2B iRFP720

    Plasmid

    #229968

    Purpose

    encodes a H2B-iRFP720 fusion contruct under the control of a dox inducible promoter

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    H2B iRFP720
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. psLIB TRE mito-mCherry

    Plasmid

    #229966

    Purpose

    encodes a mCherry targeted to mitochondria under the control of a dox inducible promoter

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    mito-mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. psLIB EF1alpha

    Plasmid

    #229954

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. psLIB TRE GTS-mTagBFP

    Plasmid

    #229969

    Purpose

    encodes a mTagBFP targeted to Golgi apparatus under the control of a dox inducible promoter

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    GTS-mTagBFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. psLIB TRE EYFP-tubulin

    Plasmid

    #229967

    Purpose

    encodes a YFP-tubulin fusion contruct under the control of a dox inducible promoter

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    EYFP-tubulin
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. psLIB TRE

    Plasmid

    #229955

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP

    Plasmid

    #204729

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

    Plasmid

    #204727

    Purpose

    Frt-inducible CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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