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pcDNA3.1 puro Nodamura B2

Plasmid

#17228

Depositor

Christopher Sullivan

Article

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Expression

Mammalian

Mutation

2 silent point mutations

Available Since

June 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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TDP-43 mRuby

Plasmid

#216225

Purpose

TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

TDP-43 (TARDBP Human)

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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AdEasier-1 cells (strain)

Bacterial Strain

#16399

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Ampicillin and Streptomycin

Species

Escherichia coli

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
AAV-CAGGS-EGFP

Plasmid

#22212

Purpose

AAV zinc finger donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

CAGGS-eGFP

Use

Zfn donor

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pN7HELIX

Plasmid

#190662

Purpose

Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB

Expression

Mammalian

Mutation

nAniI = K227M, F80K, L232K

Promoter

CMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-KRAB

Plasmid

#110820

Purpose

Conventional dCas9 repressor-dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#194703

Purpose

Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Tags

APOBEC1-HA-P2A-mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLVpuro-CMV-N-EGFP

Plasmid

#122848

Purpose

N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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EC70202

Plasmid

#209463

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 GFP shRNA

Plasmid

#30323

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

GFP shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 2, 2011

Availability

Academic Institutions and Nonprofits only

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