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pICH41276(3Uter)-tAT5G06140

Plasmid

#205404

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT5G06140

Use

Synthetic Biology

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41276(3Uter)-tAT2G26780

Plasmid

#205394

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT2G26780

Use

Synthetic Biology

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41276(3Uter)-tAT2G33730

Plasmid

#205396

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT2G33730

Use

Synthetic Biology

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41276(3Uter)-tAT3G08530

Plasmid

#205399

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT3G08530

Use

Synthetic Biology

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41276(3Uter)-tAT1G18700

Plasmid

#205387

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT1G18700

Use

Synthetic Biology

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41276(3Uter)-tAT4G34270

Plasmid

#205403

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT4G34270

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41276(3Uter)-tAT5G10630

Plasmid

#205405

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT5G10630

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41276(3Uter)-tAT5G37830

Plasmid

#205406

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT5G37830

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41276(3Uter)-tAT2G43070

Plasmid

#205397

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT2G43070

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41276(3Uter)-tAT3G07160

Plasmid

#205398

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT3G07160

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41276(3Uter)-tAT3G18480

Plasmid

#205400

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT3G18480

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41276(3Uter)-tAT1G64990

Plasmid

#205391

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT1G64990

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41276(3Uter)-tAT1G71900

Plasmid

#205393

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT1G71900

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41276(3Uter)-tAT5G60160

Plasmid

#205408

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT5G60160

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41276(3Uter)-tAT4G13780

Plasmid

#205401

Purpose

MoClo lvl0 3'UTR+Terminator part

Depositor

Jennifer Nemhauser

Article

Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

Insert

tAT4G13780

Use

Synthetic Biology

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pUT_mNF-Cas13d-Triplex

Plasmid

#227709

Purpose

LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

mCherry-P2A-RfxCas13d-Triplex

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV-d2i

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)

Plasmid

#105201

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-IL12-P2A-CCL21-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 21, 2018

Availability

Academic Institutions and Nonprofits only

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ID1GFP Donor

Plasmid

#119282

Purpose

Mouse ID1-eGFP for HR to create reporter

Depositor

Joan Massague

Article

Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

Insert

Id1GFP + homology arms (Id1 Mouse)

Use

Reporter

Tags

eGFP

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His

Plasmid

#99743

Purpose

Expresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.

Depositor

Thomas Weimbs

Article

Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

Insert

Stx3 (STX3 Human)

Tags

myc-myc-his

Expression

Mammalian

Mutation

Changed both L165 and E166 to Alanine

Promoter

CMV

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-L17-Q2L7-T2176N-UGI-Puro

Plasmid

#209647

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

L17-Q2L7-T2176N

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro

Plasmid

#209642

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

L17-FZY2-S100N

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-L17-FZY2-S100C-UGI-Puro

Plasmid

#209641

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

L17-FZY2-S100C

Expression

Mammalian

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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RNF34

Plasmid

#119937

Purpose

RING finger protein 34 expression

Depositor

Angel de Blas

Article

Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

Insert

RNF34 (Rnf34 Rat)

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only
pFL His-SDS22

Plasmid

#113512

Purpose

Expression of His-SDS22 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

SDS22 (PPP1R7 Human)

Tags

6*His

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBEG R3-L4 #BGV194

Plasmid

#48954

Purpose

For inserting selection gene between AttR3 and AttL4 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway Cloning

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
KHBD00441

Plasmid

#34544

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG33546 (gfzf Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
CXCR4-SZ158a QTY

Plasmid

#162442

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Tags

his-tag, Rho-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ146a QTY

Plasmid

#162443

Purpose

Expression in E coli and perform ligand binding in solution

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Tags

his-tag, Rho-tag

Expression

Bacterial

Mutation

All L, I, V, F in the transmembrane region mutate…

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ158a-sfGFP

Plasmid

#162446

Purpose

Expression in HEK293T cell and compete ligand singaling against full-length receptors

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Tags

sfGFP

Expression

Mammalian

Mutation

truncation from aa52 to aa257

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLentiLox7.0-CAGEc-RFP-NLS (Nrdj1)

Plasmid

#241403

Purpose

Proximity-triggered Protein Trans-Splicing between mVenus and RFP, Figure 1 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

CAGEc-RFP-NLS (Nrdj1)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-scrambled-mCherry-CAAX

Plasmid

#228738

Purpose

Membrane-tethered mCherry scrambled shRNA targeting Miro1

Depositor

Cagla Eroglu

Article

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Miro1 (Rhot1, Rhot1 Mouse, Rat)

Expression

Mammalian

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiLox7.0-mVenus-CAGEn-Caax (Nrdj1)

Plasmid

#241405

Purpose

Proximity-triggered Protein Trans-Splicing between mVenus and RFP, Figure 1 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

mVenus-CAGEn-Caax (Nrdj1)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-DiB2

Plasmid

#168801

Purpose

Fluorogen activating protein (FAP) DiB2

Depositor

Alexander Mishin

Article

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB2

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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EC71139

Plasmid

#154062

Purpose

Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

pZmUbi

Use

Synthetic Biology

Mutation

All BsaI, Esp3I and BpiI restriction sites were r…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7

Plasmid

#180315

Purpose

mammalian expression of human SEPT7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pmiR-report-hEZH2

Plasmid

#112543

Purpose

3'UTR reporter assay

Depositor

Shusheng Wang

Article

Zhou et al Mol Ther. 2014 Feb;22(2):378-389. doi: 1

Insert

EZH2 (EZH2 Human)

Use

Luciferase

Available Since

July 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-Nb80

Plasmid

#205518

Purpose

Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-Nb80

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-c/o NL4-3 Env delta CT

Plasmid

#139285

Purpose

HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

codon optimized HIV-1 Env delta cytoplasmic tail

Expression

Mammalian

Mutation

deletion of cytoplasmic tail after residue 710

Promoter

CMV IE

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MUS2-1246-vpr

Plasmid

#115841

Purpose

Encodes codon optimized 3xFLAG-SIVMUS21246 Vpr (EF070329) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMUS21246 Vpr (EF070329)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pscALPS-SIV-GRV vpr

Plasmid

#115827

Purpose

Encodes codon optimized 3xFLAG-SIVGRV Vpr (M66437) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGRV Vpr (M66437)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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