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pCASCADE-xylA

Plasmid

#158611

Purpose

Low phosphate inducible gRNA silencing the xylA promoter

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

xylA guide array

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPPC030.306

Plasmid

#171151

Purpose

Mevalonate production pathway with J306 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J306 scRNA
J3-BBa_J23117-mvaES

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J3-BBa_J23117

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSMART-ala13

Plasmid

#87174

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Use

Tags

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proD

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-ala11

Plasmid

#87172

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Use

Tags

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proA

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSMART-ala12

Plasmid

#87173

Purpose

NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

Depositor

Michael Lynch

Article

Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

Insert

NADPH Alanine Dehydrogenase (ald Synthetic)

Use

Tags

Expression

Bacterial

Mutation

D196A, L197R

Promoter

Constitutive proC

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-PhCHS-MsCHI

Plasmid

#73400

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
PhCHS
MsCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-CmCHS-CmCHI

Plasmid

#73403

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
CmCHS
CmCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Pc4CL-CmCHS-CmCHI

Plasmid

#73399

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Pc4CL
CmCHS
CmCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Pact1-ITE-Tadh1

Plasmid

#127723

Purpose

Yeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pact1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Ppre3-ITE-Teno1

Plasmid

#127724

Purpose

Yeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ppre3

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Pact1-ITE-Tdpp1

Plasmid

#127725

Purpose

Yeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pact1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-5a-Phta1-ITE-Tvma2

Plasmid

#127726

Purpose

Yeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

itaconate transporter from A. terreus

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Phta1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pfba1p-ACO-Tgpm1

Plasmid

#127731

Purpose

Yeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pfba1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Pykt6-ACO-Tprm9

Plasmid

#127732

Purpose

Yeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pykt6

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-cd-Ptef1-ACO-Tyol036w

Plasmid

#127733

Purpose

Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

aconitase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ptef1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ptef1-CSC-Tpdc1

Plasmid

#127739

Purpose

Yeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pfba1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Pzuo1-CSC-Tvma16

Plasmid

#127740

Purpose

Yeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pzuo1

Available since

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Ppfy1-CSC-Taip1

Plasmid

#127741

Purpose

Yeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Ppfy1

Available since

Availability

Academic Institutions and Nonprofits only

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CYN2/150 (E. coli Entner-Doudoroff v11)

Bacterial Strain

#71420

Purpose

This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway with an improved NADPH regeneration rate. Module is Cm resistant

Depositor

Howard Salis

Article

Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

Bacterial Resistance

Chloramphenicol

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
CYN1 (E. coli Entner-Doudoroff v1)

Bacterial Strain

#71421

Purpose

This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway (first version). The module also contains a Cm resistance marker.

Depositor

Howard Salis

Article

Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

Bacterial Resistance

Chloramphenicol

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only