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pUCBB-eGFP

Plasmid

#32548

Depositor

Claudia Schmidt-dannert

Publication

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJRH-1363 LV EF1a-CD19 IRES-EGFP

Plasmid

#201919

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD19 (CD19 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p416 25Q GPD

Plasmid

#1177

Depositor

Susan Lindquist

Publication

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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BPK2101

Plasmid

#65770

Purpose

Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized SaCas9, and SaCas9 gRNA

Use

CRISPR

Tags

NLS-3xFlag

Expression

Bacterial

Promoter

T7 (x2)

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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gag-MCP-pol

Plasmid

#211370

Purpose

Packaging plasmid for v3 PE-eVLP production

Depositor

David Liu

Publication

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-MCP-pol

Expression

Mammalian

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Human SLC KO Library

Pooled library

#132552

Purpose

Knockout library targeting human SLC proteins.

Depositor

Giulio Superti-Furga

Publication

Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. d

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pCDF-GFPplus

Plasmid

#172718

Purpose

Expression of a fast folding GFP variant GFP plus

Depositor

Tilmann Weber

Publication

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

GFP plus

Expression

Bacterial

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-pre-eGRASP(p32)

Plasmid

#111598

Purpose

An AAV vector that expresses green pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Publication

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

green pre-eGRASP(p32)

Use

AAV

Promoter

CaMKIIa

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-hAsCpf1(TYCV) (pY210)

Plasmid

#89351

Purpose

Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Publication

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CMV

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-i53

Plasmid

#92170

Purpose

recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1

Depositor

Daniel Durocher

Publication

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

i53

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

UbvG08 with I44A mutation and no terminal Glycines

Promoter

CMV

Available since

July 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pJET1.2-STOP-dsRed

Plasmid

#60944

Purpose

Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

Depositor

Frank Schnorrer

Publication

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

dsRED

Use

Unspecified

Promoter

3XP3

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pMBI

Plasmid

#17816

Depositor

Jay Keasling

Publication

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase

Use

Synthetic Biology

Expression

Bacterial

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA3-sACE2(WT)-8his

Plasmid

#149268

Purpose

Mammalian expression plasmid for his-tagged sACE2

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Short GSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry

Plasmid

#85431

Purpose

Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human Tbet

Use

Lentiviral

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL-c5x_UnaG

Plasmid

#163125

Purpose

A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

Depositor

Julie Biteen

Publication

Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

Insert

UnaG

Tags

His and MBP

Expression

Bacterial

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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DRH322: AAV-CaMKIIa-QuasAr2-mO2

Plasmid

#51696

Purpose

AAV production of QuasAr2 fluorescent reporter

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

AAV

Tags

mOrange2

Promoter

CaMKIIa

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-HF-BE3

Plasmid

#87439

Purpose

Expresses HF-BE3 with C-terminal NLS in mammalian cells

Depositor

David Liu

Publication

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Bacillus phage PBS2, Rat, Human)

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6e

Plasmid

#207855

Purpose

Mammalian expression of PE6e prime editor

Depositor

David Liu

Publication

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6e

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, K918A, K775R)

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-CXCL12-sfGFP

Plasmid

#98961

Purpose

Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

Depositor

Erik Procko

Publication

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCL12 (CXCL12 Human)

Tags

Superfolder GFP

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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