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We narrowed to 2,375 results for: pcas9

Showing: 841 - 860 of 2375 results

  1. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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  2. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  3. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-90684

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  4. gTBEv4

    Plasmid
    #220620
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220620-plasmid-map-sequence-id-436970

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  5. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

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  6. pxCas9CR4

    Plasmid
    #111656
    Purpose
    PCas9CR4 with the xCas9 mutations allowing NG PAM sites
    Depositor
    Christopher Reisch
    Insert
    TetR and xCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Bacterial
    Mutation
    xCas9 mutations
    Promoter
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111656-plasmid-map-sequence-id-217189

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  7. pSJX022

    Plasmid
    #232325
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and cat
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pvan
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232325-plasmid-map-sequence-id-478586

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  8. pSJX016

    Plasmid
    #232324
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pvan
    Available Since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232324-plasmid-map-sequence-id-476126

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  9. pIF1079

    Plasmid
    #237445
    Purpose
    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.
    Depositor
    IIya Finkelstein
    Article
    Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/
    Inserts
    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, H1, and U6
    Available Since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    237445-plasmid-map-sequence-id-476973

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  10. pSJX024

    Plasmid
    #232327
    Purpose
    Genome editing in Caulobacter crescentus
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and cat
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pxyl
    Available Since
    June 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232327-plasmid-map-sequence-id-476136

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  14. pSJX067

    Plasmid
    #232328
    Purpose
    Genome editing in Agrobacterium fabrum
    Depositor
    Wei Zhao
    Article
    Sun et al bioRxiv 2024.12.02.626314
    Inserts
    sgRNA
    SpCas9M
    Use
    CRISPR
    Tags
    H-arms and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    PJ23119 and Pvan
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232328-plasmid-map-sequence-id-478585

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  15. pYPQAT31

    Plasmid
    #223403
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223403-plasmid-map-sequence-id-454164

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  16. pYPQAT57

    Plasmid
    #223429
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223429-plasmid-map-sequence-id-453502

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  17. pYPQAT56

    Plasmid
    #223428
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223428-plasmid-map-sequence-id-453528

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  18. pYPQAT59

    Plasmid
    #223431
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223431-plasmid-map-sequence-id-453576

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  19. pYPQAT60

    Plasmid
    #223432
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223432-plasmid-map-sequence-id-475539

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  20. pYPQAT58

    Plasmid
    #223430
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223430-plasmid-map-sequence-id-454181

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Showing: 841 - 860 of 2375 results