We narrowed to 73,509 results for: LAS

1. pRSET.HaloTag.v7.CoOp.Cys-less

   Plasmid

   #195487

   Purpose

   Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C61T, C262V

   Promoter

   T7

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. mT2A-td-sfGFP-FNF

   Plasmid

   #182339

   Purpose

   Recombineering cassette plasmid for T2A-td-sfGFP-FNF

   Depositor

   Mario Capecchi

   Insert

   T2A-td-sfGFP-FNF
   

   Use

   Mouse Targeting; Flp / frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Z-MATR3-4

   Plasmid

   #166558

   Purpose

   scFv of a human scaffold targeting Matrin-3. Antigen coverage aa 390-578 of 847

   Depositor

   Susanne Gräslund

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST26-his-hSFRP1

   Plasmid

   #189539

   Purpose

   Protein expression of SFRP1 in mammalian cells.

   Depositor

   Haian Fu

   Insert

   secreted frizzled related protein 1 (SFRP1 Human)
   

   Use

   Tags

   his

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPK719

   Plasmid

   #38149

   Depositor

   Patricia Kuwabara

   Insert

   cbr-unc-119::FRT*::galk::FRT* (Cbr-unc-119 Nematode)
   

   Use

   Recombineering

   Tags

   Expression

   Worm

   Mutation

   Promoter

   unc-119

   Available since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV Tor1a-Myc

   Plasmid

   #175134

   Purpose

   Lentiviral expression of Myc-tagged mouse Tor1a

   Depositor

   Larry Gerace

   Insert

   Tor1a (Tor1a Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS ZfHoxb1a

   Plasmid

   #27519

   Depositor

   Victoria Prince

   Insert

   Hoxb1a (hoxb1a Zebrafish)
   

   Use

   Riboprobe synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pUPD2 nickase D10A Cas9 (GB1690)

   Plasmid

   #160587

   Purpose

   Nickase D10A Cas9 for Nt and Ct fusion.

   Depositor

   Diego Orzaez

   Insert

   nickase D10A Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CamKIIa-C1V1::FusionRed::Kv2.1

   Plasmid

   #102771

   Purpose

   "Activate neurons with 2p stimulation"

   Depositor

   Karel Svoboda

   Insert

   C1V1::FusionRed::Kv2.1
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1 traficking domain

   Expression

   Mutation

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPIN3A

    Plasmid

    #74656

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPIN3A (SPIN3 Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pLenti6.3_HES2

    Plasmid

    #124070

    Purpose

    HES2 with normal stop codon

    Depositor

    J. Chad Brenner

    Insert

    HES2 (HES2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. A-WDR5-1

    Plasmid

    #166515

    Purpose

    scFv of a human scaffold targeting WD repeat domain 5. Antigen coverage aa 1-334 of 334.

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE.puro/TO/Flag/mKO2

    Plasmid

    #110353

    Purpose

    Doxycycline inducible mammalian retroviral expression of mKO2 with FLAG tag

    Depositor

    Sandra Martha Gomes Dias

    Insert

    monomeric Kusabira Orange 2
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    gag

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS ZfHoxa2b

    Plasmid

    #27514

    Depositor

    Victoria Prince

    Insert

    Hoxa2b (hoxa2b Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

15. EGFP-hPMCA4bct125

    Plasmid

    #47594

    Depositor

    Emanuel Strehler

    Insert

    PMCA4bct125 (ATP2B4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    125 C terminal AA deleted

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

16. PP-SRM

    Plasmid

    #25110

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PF11_0301:C22904 (PF11_0301 Plasmodium falciparum)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. PP-ClpR

    Plasmid

    #32876

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ClpR (PF3D7_1436800 Plasmodium Falciparum)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. psicheck_NDUFA6_3UTR

    Plasmid

    #65886

    Purpose

    Luciferase assay vector, assessing regulation of NDUFA6 3' UTR

    Depositor

    Thomas Tuschl

    Insert

    NDUFA6 (NDUFA6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pBS ZfHoxb2a

    Plasmid

    #27521

    Depositor

    Victoria Prince

    Insert

    Hoxb2a (hoxb2a Zebrafish)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

20. Py-PPI (PY00382)

    Plasmid

    #25567

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Py-PPI (PY00382) (PY00382 Plasmodium yoelii)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits