We narrowed to 18,438 results for: JUN

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pCMV-ACBEmax

Plasmid

#139106

Purpose

Mammalian ACBEmax expression vector (pSI-632)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTet01

Plasmid

#78283

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMG81-MBP-t-M.MpeI-N374K-His

Plasmid

#197985

Purpose

Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.

Depositor

Rahul Kohli

Article

Wang et al Nat Chem Biol. 2023 Jun 15. doi: 10.1038

Insert

MBP-t-M.MpeI-N374K-His

Tags

His and MBP

Expression

Bacterial

Mutation

M.MpeI-N374K

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P2

Plasmid

#64786

Purpose

Luciferase expression driven by miR-34a promoter P2

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P2 (MIR34A Human)

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pIE-TGFB2-H6

Plasmid

#128500

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Tags

His tag (6x)

Expression

Insect

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR3c-R248C

Plasmid

#45712

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR3 IIIc (FGFR3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R248C

Available Since

June 12, 2013

Availability

Academic Institutions and Nonprofits only
pXPE

Plasmid

#15284

Depositor

Ron Vale

Article

Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

Insert

ubiquitous kinesin heavy chain (KIF5B Human)

Expression

Bacterial

Mutation

The insert is missing the 1st 180 bp of 5' u…

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pHelper-T7IS1

Plasmid

#140631

Purpose

Expresses ShCAST under the T7 promoter and an empty sgRNA

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-Hs4EHP_AE

Plasmid

#148588

Purpose

Mammalian Expression of Hs4EHP

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP (EIF4E2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187948

Purpose

ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

ER50-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVTHM-shRnd3

Plasmid

#86437

Purpose

2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFP

Depositor

Violaine Moreau

Article

Grise et al Hepatology. 2012 Jun;55(6):1766-75. doi:

Insert

shRNA targeting RND3 (RND3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-NpHR3.3-EYFP

Plasmid

#137151

Purpose

Viral expression of NpHR3.3-EYFP

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

NpHR3.3-EYFP

Use

AAV

Mutation

W179F

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Alpha MHC-DN.JNK2

Plasmid

#51931

Purpose

mammalian expression of dominant negative JNK2

Depositor

Jeffery Molkentin

Article

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

DN JNK2 (Mapk9 Mouse)

Tags

HA

Expression

Mammalian

Mutation

dominant negative: the dual phosphorylation moti…

Promoter

cardiac-specific α-myosin heavy chain 5.5 kb pro…

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only
pMRXIP-GFP-CERT(PHD)(W33A)

Plasmid

#221761

Purpose

Stable expression of GFP-CERT(PHD)(W33A) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

CERT (CERT1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–116, mutated 33W to 33A

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-548e-5p

Plasmid

#103634

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-548e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-548e-5p target (MIR548E Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187950

Purpose

ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Kaede

Plasmid

#219433

Purpose

AAV expression vector for Kaede

Depositor

Yoshiho Ikeuchi

Article

Martins-Costa et al Cell Stem Cell. 2024 Jun 6;31(6):866-885

Insert

Kaede

Use

AAV

Promoter

CAG

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-His10-SHP1_PTPase (243-541aa)

Plasmid

#180812

Purpose

Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SHP1_PTPase (243-541aa) (PTPN6 Human)

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAd-Track HA PGC-1 alpha

Plasmid

#14427

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pAG426GAL-EGFP

Plasmid

#166143

Purpose

EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

EGFP

Expression

Yeast

Promoter

GAL1

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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