We narrowed to 166,303 results for: addgene

1. U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

   Plasmid

   #194283

   Purpose

   EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Promoter

   EF1a

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDK637

   Plasmid

   #134203

   Purpose

   bacterial expression of human CENP-N 1-240

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   CENP-N 1-240-His (CENPN Human)
   

   Expression

   Bacterial

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-mir144~451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164793

   Purpose

   Express miR-144~451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-144~451 (MIR451A, MIR144 Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDR366 RNF2

   Plasmid

   #47509

   Purpose

   human gRNA expression vector targeting RNF2

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-RNF2 (RNF2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCL.175

   Plasmid

   #184996

   Purpose

   Express -Eco1 HEK3 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   HEK3 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP787_SnoopCatcher-EZH2-FL-CO

   Plasmid

   #211780

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-DNMT3A
   

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCL.177

   Plasmid

   #184998

   Purpose

   Express -Eco1 EMX1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   EMX1 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA2_ASCL1

   Plasmid

   #64130

   Purpose

   Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1_ASCL2 (ASCL1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC_C_HXK1

   Plasmid

   #111668

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   HXK1 (HK1 Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiPGK Puro DEST JNKKTR EE Clover

    Plasmid

    #90239

    Purpose

    Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter (EE mutant)
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti_CRISPRi_sgNeg1

    Plasmid

    #166998

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 shJUND puro

    Plasmid

    #58698

    Purpose

    Lentiviral shRNA vector for knockdown of human JUND

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    shJUND (JUND Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pX459-sgRNA2-RPB1-N-term

    Plasmid

    #195111

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against first exon of RPB1 (N-terminal)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVC299 VEGF Site#2

    Plasmid

    #47506

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 2 (VEGFA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICE-HA-CtIP-siR-WT

    Plasmid

    #82030

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    CtIP (RBBP8 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutations making the cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICSL11055

    Plasmid

    #68252

    Purpose

    Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b-BE3∆UGI

    Plasmid

    #96943

    Purpose

    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

    Depositor

    Daesik Kim

    Article

    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

    Insert

    BE3∆UGI
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCL.180

    Plasmid

    #185001

    Purpose

    Express -Eco1 AAVS1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    AAVS1 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CMV-cGAS R71/75E-HA

    Plasmid

    #130919

    Purpose

    Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    human cGAS R71/75E
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 71 and 75 mutated to glutamate

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Runx1_pCSdest

    Plasmid

    #53802

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    Runx1 (RUNX1 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits