167,825 results

1. MSCB-hDot1Lmut

   Plasmid

   #74174

   Purpose

   For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

   Depositor

   Yi Zhang

   Article

   Okada et al Cell. 2005 Apr 22;121(2):167-78.

   Insert

   hDot1L (DOT1L Human)
   

   Use

   Retroviral

   Tags

   HA

   Mutation

   hDot1L 163 GSG to RCR mutation*

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

2. LgBit-NbV5

   Plasmid

   #201476

   Purpose

   NanoBit detection of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   LgBit-NbV5
   

   Tags

   LgBit

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-5xGCN4-P2A-BFP

   Plasmid

   #184438

   Purpose

   dCas9 fused with 5 copies of GCN4 epitope

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   dCas9-5xGCN4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HMA-APOBEC1-Nb

   Plasmid

   #226012

   Purpose

   For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

   Depositor

   Eugene Yeo

   Article

   Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Tags

   His-MBP and antiRabbit-nanobody

   Expression

   Bacterial

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIZ-Flag6His-BmAgo3

   Plasmid

   #50559

   Purpose

   Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

   Depositor

   Yukihide Tomari

   Article

   Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

   Insert

   BmAgo3
   

   Tags

   6xHIS and Flag

   Expression

   Insect

   Promoter

   OpIE2

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE30-HyPer7

   Plasmid

   #141071

   Purpose

   Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   Hisx6

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGolt-mCherry / pGolt3-mCherry

   Plasmid

   #73297

   Purpose

   Visualization of Dendritic Golgi Satellite

   Depositor

   Michael Kreutz

   Article

   Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

   Inserts

   ER export signal of Scap
   ER export signal of Scap
   TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
   

   Tags

   ER export and mCherry

   Expression

   Mammalian

   Promoter

   CMV immediate early promoter

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCMV-LCMV-Arm53b

   Plasmid

   #15796

   Depositor

   Miguel Sena-Esteves

   Article

   Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

   Insert

   LCMVArm53b envelope glycoprotein
   

   Expression

   Mammalian

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. NW11

   Plasmid

   #133443

   Purpose

   Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW11
   

   Expression

   Bacterial

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3TS-RfxCas13d-NLS-HA

    Plasmid

    #141321

    Purpose

    Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos, Ariel Bazzini

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d (NLS-RfxCas13d-NLS)
    

    Use

    CRISPR

    Tags

    HA and NLS

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-NFAT1(4-460)-GFP

    Plasmid

    #11107

    Purpose

    Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

    Depositor

    Anjana Rao

    Article

    Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Tags

    3x HA and GFP

    Expression

    Mammalian

    Mutation

    Contains amino acids 4-460 of NFAT1; L78P compare…

    Promoter

    CMV

    Available Since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE3G-EBF1

    Plasmid

    #96965

    Purpose

    Dox-inducible expression of Ebf1 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    Ebf1 (Ebf1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC18

    Plasmid

    #50004

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

    Plasmid

    #120563

    Purpose

    Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FH-Tet2-pEF

    Plasmid

    #41710

    Purpose

    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

    Insert

    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

    Plasmid

    #219782

    Purpose

    CaST-IRES for HEK cell expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFH3.3_Cre-MS2x12

    Plasmid

    #205559

    Purpose

    Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Cre
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-Blasticidin

    Plasmid

    #75085

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

    Plasmid

    #169283

    Purpose

    Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein

    Depositor

    Dirk Heckl

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-mDlx-NLS-mRuby2

    Plasmid

    #99130

    Purpose

    An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    NLS-mRuby2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    mDLX5/6

    Available Since

    Aug. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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