We narrowed to 14,156 results for: ckb

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pDEST-Spe3-RfA-Venus

Plasmid

#186372

Purpose

Destination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCpf1

Plasmid

#122185

Purpose

Bacterial genome editing plasmid with kanamycin resistance marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S

Plasmid

#149638

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, empty

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pKIKOrbsARCm

Plasmid

#46765

Purpose

E.coli genomic integration rbsAR locus

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pKIKOrbsARKm

Plasmid

#46768

Purpose

E.coli genomic integration rbsAR locus

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCfB3405

Plasmid

#106166

Purpose

EasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT58_X2site

Plasmid

#177705

Purpose

Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ142-ZmUbi-tRNA

Plasmid

#158578

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pA-HYG OSCAR

Plasmid

#29640

Depositor

David_L Andrews, Scott Gold

Article

Paz et al Fungal Genet Biol. 2011 Jul;48(7):677-84

Type

Empty backbone

Available Since

Sept. 19, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR4-HA-myc-nuc (w557-1)

Plasmid

#20025

Purpose

Gateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

HA, Myc, and NLS

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pST-KiT

Plasmid

#44562

Purpose

for integrative inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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PL6-CS-WR

Plasmid

#122512

Purpose

construction for sgRNA transcription with Plasmodium falciparum U6 promotor

Depositor

Lubin Jiang

Article

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Type

Empty backbone

Use

CRISPR

Promoter

PfU6

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pYL322d1/Vn-C

Plasmid

#183154

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2

Plasmid

#124176

Purpose

piggyBac-based Dox-inducible expression (moderate)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pWN172

Plasmid

#215291

Purpose

Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Tags

His

Expression

Bacterial

Promoter

J23113

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMH-Flag-HA

Plasmid

#101766

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA & Flag-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

Flag-HA

Expression

Mammalian

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-C-moxGFP-Zeo

Plasmid

#207732

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCpf1-sp

Plasmid

#122186

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pelBHisTEV

Plasmid

#157741

Purpose

empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites

Depositor

Debra Hansen

Type

Empty backbone

Tags

PelB signal sequence + His10-tag + TEV protease c…

Expression

Bacterial

Promoter

T7lac

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2

Plasmid

#124174

Purpose

piggyBac-based Dox-inducible expression (weak)

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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