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pMpGWB123

Plasmid

#68577

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-TEX264ΔLIR-GFP

Plasmid

#201926

Purpose

For lentiviral transduction of TEX264ΔLIR-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

TEX264 (TEX264 Human)

Use

Lentiviral

Mutation

F273A

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.Kif1a 1-396.GFP

Plasmid

#45058

Depositor

Gary Banker, Marvin Bentley

Article

Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

Insert

Kif 1a (Kif1a Rat)

Tags

GFP

Expression

Mammalian

Mutation

motor domain aa 1-396

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only
PL-SIN-PGK-EGFP

Plasmid

#21316

Purpose

Control Lentiviral vector with ubiquitous PGK promoter, expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d

Plasmid

#215822

Purpose

Express CBE6d (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-SpCas9-2xUGI (cas9 )

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pQTEV-MPP6

Plasmid

#34806

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

membrane protein, palmitoylated 6 (PALS2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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NPM1_mNeonGreen_Donor

Plasmid

#178093

Purpose

HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

NPM1-mNeonGreen HDR donor

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-TAX1BP1

Plasmid

#175761

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSiMPlk_N + pSiMPlk_C

Plasmid

#134310

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

kanR_gp41-1 N-intein + kanR_gp41-1 C-intein

Expression

Bacterial

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAC150-KEIMA-RAMP4

Plasmid

#201929

Purpose

PiggyBac Vector expressing KEIMA-RAMP4

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

RAMP4 (SERP1 Human)

Expression

Mammalian

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCx55.5-TrpR

Plasmid

#86373

Purpose

for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

TrpR

Use

Zebrafish

Promoter

cx55.5

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1

Plasmid

#175766

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC2

Plasmid

#173468

Purpose

Human PGRMC2 containing an N-terminal FLAG tag

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC2 (PGRMC2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET YBX-1

Plasmid

#13035

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

Insert

YBX1 (YBX1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only
YSBE 524 : pNEO_mCherry

Plasmid

#92084

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, Neo selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

mCherry

Use

tagging proteins in cryptococcus neoforman

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB103

Plasmid

#68557

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRTC2-puro

Plasmid

#190567

Purpose

Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.

Depositor

Anthony Furano

Article

Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

L1 (L1.3) with reporter cassette

Expression

Mammalian

Promoter

SV40

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH-POLY

Plasmid

#64915

Purpose

Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

Depositor

Huiwang Ai

Article

Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

Inserts

Polyspecific aminoacyl-tRNA Synthetase
Amber Suppression tRNA

Tags

None

Expression

Mammalian

Promoter

CMV and U6/H1

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAC150-FAM134C-GFP

Plasmid

#201932

Purpose

PiggyBac Vector expressing FAM134C-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

FAM134C (RETREG3 Human)

Expression

Mammalian

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pQTEV-PSMD11

Plasmid

#31333

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

Plasmid

#159899

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | tagRFP | cbr-tbb-2 UTR

Expression

Worm

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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PL-SIN-Nanog-EGFP

Plasmid

#21321

Purpose

Lentiviral vector, expresses EGFP under Nanog promoter

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Nanog promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-basP:fLuc-TK:rLuc

Plasmid

#138369

Purpose

Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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HP138-neo

Plasmid

#134247

Purpose

Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

Contains Cas9

Use

CRISPR

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB102

Plasmid

#68556

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB110

Plasmid

#68564

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DO(FAS)-GCaMP6s

Plasmid

#110135

Purpose

Cre-off expression of GCaMP6s

Depositor

Bernardo Sabatini

Article

Markowitz et al Cell. 2018 May 10. pii: S0092-8674(18)30

Insert

GCaMP6s

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB301

Plasmid

#68629

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB111

Plasmid

#68565

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB105

Plasmid

#68559

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB326

Plasmid

#68654

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

No Promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB108

Plasmid

#68562

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC103-PBNeo-U6-sgRNA-Expression

Plasmid

#48228

Purpose

sgRNA expression on PiggyBac vector with Neo-selectable marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET14-GFP30_Encap

Plasmid

#86406

Purpose

Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 30aa CLP tag
Encapsulin

Tags

6x Histidine

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB106

Plasmid

#68560

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM231 - [Pmlc-1 | gfp | cbr-tbb-2 UTR]

Plasmid

#159897

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | gfp | cbr-tbb-2 UTR

Expression

Worm

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PL-SIN-EOS-C(3+)-EGFP

Plasmid

#21318

Purpose

Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

EOS-C(3+) promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-WIPI2d-TEVcs-TSF

Plasmid

#171420

Purpose

For the purifcation of GFP- WIPI2d protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

WIPI2d (WIPI2 Human)

Expression

Mammalian

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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