We narrowed to 166,303 results for: addgene

1. pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

   Plasmid

   #63886

   Purpose

   An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

   Depositor

   Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   ER-localized GCaMP3(wt)
   

   Use

   AAV

   Promoter

   human SYN1

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Zebrafish-gRNA-0001

   Plasmid

   #42241

   Purpose

   gRNA targeted to zebrafish gene apoea

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   gRNA-apoea (apoea Zebrafish)
   

   Use

   CRISPR; Zebrafish expression

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

3. gh176

   Plasmid

   #106837

   Purpose

   expression of gRNA targeting HAS2

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   HAS2 (HAS2 Human)
   

   Expression

   Mammalian

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. gh175

   Plasmid

   #106836

   Purpose

   expression of gRNA targeting HAS1

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   HAS1 (HAS1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. gh177

   Plasmid

   #106827

   Purpose

   expression of gRNA targeting HAS3

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   HAS3 (HAS3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PMYT1_HUMAN_D0

   Plasmid

   #79687

   Purpose

   This plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   PMYT1
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. gh267

   Plasmid

   #106866

   Purpose

   expression of gRNA targeting ALG13

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   ALG13 (ALG13 Human)
   

   Expression

   Mammalian

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe puro human Frataxin

   Plasmid

   #11505

   Depositor

   Ronald Kahn

   Article

   Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

   Insert

   Frataxin (FXN Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

9. FB002

   Plasmid

   #119676

   Purpose

   Terminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Terminator tub
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FB008

    Plasmid

    #119706

    Purpose

    Terminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Terminator trpC
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFETCh_ARID4B

    Plasmid

    #101072

    Purpose

    Donor vector for 3' FLAG tag of human ARID4B

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ARID4B homology arms (ARID4B Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. JARID2-KO-gRNA-1

    Plasmid

    #131328

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

13. JARID2-KO-gRNA-2

    Plasmid

    #131327

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

14. pIRES-EGFP-TPD52

    Plasmid

    #172460

    Purpose

    Expression of untagged Tumor Protein D52 (TPD52) and GFP

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD52 (TPD52 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-sgRNA_Ago1_3

    Plasmid

    #73535

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

16. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124544

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. 8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124540

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124538

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PRPF4B gRNA (BRDN0001146164)

    Plasmid

    #76428

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRPF4B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRPF4B
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. 49535-sgFMR1_E17A

    Plasmid

    #157782

    Purpose

    Expresses a sgRNA targeting stop codon of human FMR1 gene

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1 (FMR1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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