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pHH0103_STAM-1/1

Plasmid

#91428

Purpose

Protein expression and purification of human SH3 domain construct STAM-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAM-1/1 (STAM Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGI vpx

Plasmid

#115815

Purpose

Encodes codon optimized 3xFLAG-SIVAGI Vpx (HM803690) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGI Vpx (HM803690)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-DRL vpx

Plasmid

#115814

Purpose

Encodes codon optimized 3xFLAG-SIVDRL Vpx (KM378563) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDRL Vpx (KM378563)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MNE vpx

Plasmid

#115813

Purpose

Encodes codon optimized 3xFLAG-SIVMNE Vpx (U79412) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMNE Vpx (U79412)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Nap4-SH2

Plasmid

#46456

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nap4 (SOCS7 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 395-514

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Nsp1-SH2

Plasmid

#46459

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp1 (SH2D3A Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 3-119

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pHH0103_PACSIN3-1/1

Plasmid

#91335

Purpose

Protein expression and purification of human SH3 domain construct PACSIN3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN3-1/1 (PACSIN3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJB3

Plasmid

#19552

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 3 (DNAJB3 Mouse)

Tags

His

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pTAtUbq3 (GB0219)

Plasmid

#68192

Purpose

Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq3 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0011

Plasmid

#117530

Purpose

Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-xCas9 3.7 (no stop codon)

Use

CRISPR

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PFA0445w-bio-His

Plasmid

#50811

Purpose

Expresses enzymatically monobiotinylated full length PFA0445w

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFA0445w

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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PF10_0166-bio-His

Plasmid

#50801

Purpose

Expresses enzymatically monobiotinylated full length PF10_0166

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PF10_0166

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pGEM-rat Fcepsilon RI gamma

Plasmid

#8375

Depositor

Jean Kinet

Article

Blank et al Nature 1989 Jan 12;337(6203):187-9.

Insert

rat Fc epsilon RI gamma cDNA (Fcer1g Rat)

Use

Beta-gal

Expression

Bacterial

Available Since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
HOXD13 (chicken) HIS-tag pET

Plasmid

#8555

Depositor

Corey Largman

Article

Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

Insert

HOXD13 (HOXD13 )

Tags

His and T7

Expression

Bacterial

Mutation

contains 10 nt 5' to ATG start; makes prote…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
vsv-Sgo1 N61I-GFP

Plasmid

#108497

Purpose

expression of vsv-Sgo1 N61I-EGFP (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

no PP2A binding N61I

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 R112C/T180C

Plasmid

#66182

Purpose

Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C, T180C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 E105C/L171C

Plasmid

#66178

Purpose

Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

E105C, L171C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pISH-Meis1

Plasmid

#74348

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Meis1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Meis homeobox 1 (Meis1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR 19b-3p

Plasmid

#82442

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-19-3p

Mutation

Complementary region for hsa-miR-19-3p as sensor

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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Dom34(265-386)

Plasmid

#19102

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only