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PCYOX1L-bio-His

Plasmid

#53410

Purpose

Expresses full-length Prenylcysteine oxidase-like precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PCYOX1L (PCYOX1L Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MEKK1 KD

Plasmid

#12180

Depositor

Gary Johnson

Article

Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Insert

MEKK1 (Map3k1 Mouse)

Tags

His and Xpress

Expression

Mammalian

Mutation

Kinase dead: K1253M; V1467L

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
GFP-DRD3

Plasmid

#24098

Depositor

Jean-Michel Arrang

Article

Jeanneteau et al Mol Biol Cell. 2004 Feb . 15(2):696-705.

Insert

DRD3 (DRD3 Human)

Tags

alpha7 nicotinic signal peptide and eGFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pSPneogRNA241510+MT

Plasmid

#84292

Purpose

Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA

Use

CRISPR; Leishmania donovani

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-STIL

Plasmid

#136795

Purpose

Mammalian expression of the centrosomal protein STIL N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-STIL (STIL Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-mNectarine

Plasmid

#21717

Purpose

Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

Depositor

Robert Campbell

Article

Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

Insert

mNectarine fluorescent proteins

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pspCas9-HF-tetra-com-vector

Plasmid

#132555

Purpose

Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Inserts

High fidelity Cas9
sgRNA scaffold with com replacing the Tetraloop

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1750-2549 aa)

Plasmid

#21747

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1750-2549) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1750-2549

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pSMALB

Plasmid

#161786

Purpose

Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

Bidirectional minhCMV and SFFV

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFPLaminA-N195K

Plasmid

#32708

Depositor

Wendy Bickmore

Article

Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

Insert

Lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Mutation

N195K

Promoter

CMV

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFX-iRFP-Rab5

Plasmid

#174456

Purpose

Express iRFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with early endosome-targeting sequence

Tags

small GTPase (Rab5)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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HSC1-GiP

Plasmid

#58254

Purpose

This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TAT-1-101-FLAG

Plasmid

#35981

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV1 101 amino acids

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pDD315 (mTurquoise2^SEC^2xHA)

Plasmid

#73343

Purpose

mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

Insert

mTurquoise2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

2xHA and C. elegans codon-optimized mTurquoise2

Expression

Worm

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-2A-3xFLAG-XBP1u_pause-2A

Plasmid

#159583

Purpose

Luciferase reporter for ribosome pausing

Depositor

Shintaro Iwasaki

Article

Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

Inserts

XBP1u pause site
EIF2S3 5UTR

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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MTK2_023

Plasmid

#123715

Purpose

Encodes pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pR26 iBFP Dest

Plasmid

#74284

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.7

Plasmid

#78176

Purpose

Expresses rsGreen0.7 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.7

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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PM-FRB-CFP

Plasmid

#67517

Purpose

FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

mTOR (MTOR Human)

Tags

CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

Expression

Mammalian

Mutation

FRB domain only (residues 2019–2114)

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-GFPLaminA-L85R

Plasmid

#32707

Depositor

Wendy Bickmore

Article

Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

Insert

Lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Mutation

L85R

Promoter

CMV

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only