166,671 results

1. pGPTVII-Bar-U-MatryoshCaMP6s

   Plasmid

   #100024

   Purpose

   Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRRL_SRSF2_WT_mCherry

   Plasmid

   #84020

   Purpose

   expression of SRSF2 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

   Insert

   SRSF2 (SRSF2 Human)
   

   Use

   Lentiviral

   Tags

   2TA-mCherry and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. FTL

   Plasmid

   #100750

   Purpose

   Part of FerriTag expression system. Untagged Ferritin light chain in mammalian expression vector.

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   FTL (FTL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSII-EF/mt-(n1)StayGold

   Plasmid

   #185823

   Purpose

   A soluble photostable fluorescent marker of the mitochondrial matrix. The StayGold gene has codon optimization for mammalian expression.

   Depositor

   Atsushi Miyawaki

   Article

   Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103

   Insert

   mt-(n1)StayGold
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-Actin-7

   Plasmid

   #56421

   Purpose

   Localization: Actin, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Actin (ACTB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 23, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRE-Slow-FT

   Plasmid

   #31915

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Slow-FT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

   Promoter

   PTight Tet-responsive PminCMVd

   Available Since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-N EBNA1

   Plasmid

   #37954

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   EBNA1 (EBNA-1 H. Herpesvirus 4 (EBV))
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   PKG

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (AAV9)

   Viral Prep

   #100848-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Promoter

   Syn

   Tags

   None

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro

    Plasmid

    #210857

    Purpose

    Lentiviral expression vector encoding FIP200 mutant-mCherry

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    V44D/V314D/L324D/R543D/R548D

    Promoter

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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