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Plasmid#104036PurposeDonor vector for 3' FLAG tag of human GATAD1DepositorInsertGATAD1 homology arms (GATAD1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX330_MED13_iso1_1
Plasmid#135751PurposeEncodes gRNA for 3' target of human MED13_iso1DepositorInsertMED13_iso1 gRNA (MED13 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPG_2
Plasmid#86345PurposeEncodes gRNA for 3' target of human CEBPGDepositorInsertgRNA against CEBPG (CEBPG Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBE2QL1-GFP
Plasmid#124667PurposeExpresses UBE2QL1 with C-terminal GFP tag in mammalian cellsDepositorInsertUBE2QL1 (UBE2QL1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(369-598)
Plasmid#135438PurposeExpresses SFPQ 369-598 with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC1-pCR8-dCas9VP48
Plasmid#48214PurposedCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP48 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP48ExpressionMutationD10A;H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_KLF9
Plasmid#86286PurposeDonor vector for 3' FLAG tag of human KLF9DepositorInsertKLF9 homology arms (KLF9 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_KLF9_2
Plasmid#86347PurposeEncodes gRNA for 3' target of human KLF9DepositorInsertgRNA against KLF9 (KLF9 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_KLF9_1
Plasmid#86346PurposeEncodes gRNA for 3' target of human KLF9DepositorInsertgRNA against KLF9 (KLF9 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_GATAD1_2
Plasmid#104043PurposeEncodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFPDepositorInsertGATAD1 gRNA (GATAD1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_GATAD1_1
Plasmid#104042PurposeEncodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFPDepositorInsertGATAD1 gRNA (GATAD1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3-2X
Plasmid#110835PurposeCMV expression vector for BE3-2X construct (not codon optimized)DepositorInsertBE3-2X
UseTagsExpressionMammalianMutation2 tandem NLS sequences in the XTEN linkerPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL417
Plasmid#62822PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 terminator + ColE1DepositorInsertsgfp
micC leader
hfq leader
UseSynthetic BiologyTagsompC leader and st7 RBSExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYO1124
Plasmid#235725PurposeExpression of human UVRAG (1-464) fused to human ATG14L BATS (413-492) in mammalian cellsDepositorInsertUVRAG(1-464) fused to ATG14L BATS domain (413-492)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pLJC2-Blast-DHFR-3xFLAG
Plasmid#233892Purposelentiviral overexpression vector for DHFRDepositorInsertDHFR (DHFR Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-Blast-DHFR
Plasmid#233893Purposelentiviral overexpression vector for DHFRDepositorInsertDHFR (DHFR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
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FAM177A1-Halo
Plasmid#224591PurposeFor expression of FAM177A1-Halo in mammalian cellsDepositorInsertFAM177A1 (FAM177A1 Human)
UseTagsHALOExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgMTHFD1
Plasmid#217436PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human MTHFD1DepositorInsertsgRNA targeting MTHFD1 (MTHFD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-TK2-3xFLAG
Plasmid#217427PurposeLentiviral overexpression of human TK2DepositorInsertTK2 (TK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationInsert sequence is a codon-optimized gBLOCK (IDT)PromoterCMVAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgTYMS
Plasmid#217430PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human TYMSDepositorInsertsgRNA targeting TYMS (TYMS Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgTK2_4
Plasmid#217431PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human TK2DepositorInsertsgRNA 4 targeting TK2 (TK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgTK2_5
Plasmid#217432PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human TK2DepositorInsertsgRNA 5 targeting TK2 (TK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgSHMT2
Plasmid#217435PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2DepositorInsertsgRNA targeting SHMT2 (SHMT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG
Plasmid#200792PurposeMammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutationΔ954-1027PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG
Plasmid#200798PurposeMammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutation411-680PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
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FAM177A1-SNAP
Plasmid#224592PurposeFor expression of FAM177A1-SNAPTag in mammalian cellsDepositorInsertFAM177A1 (FAM177A1 Human)
UseTagsSNAPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only