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Showing: 1801 - 1840 of 8502 results
  1. pV1090

    Plasmid
    #111427
    Purpose
    gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)
    Depositor
    Insert
    sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pMLS256

    Plasmid
    #73715
    Purpose
    SapTrap destination vector for building combined sgRNA expression + repair template vectors
    Depositor
    Insert
    pU6::sgRNA
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    SapI insertion site
    Promoter
    C. elegans U6 snRNA pol III promoter
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. pWT062d

    Plasmid
    #107911
    Purpose
    W2m.2-4 plasmid. Contains H1-TetR-sgRNA B and is ppart of the CAMERA2m system for cellular recording in mammalian cells
    Depositor
    Insert
    H1-TetR-sgRNA B
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pWT062e

    Plasmid
    #107910
    Purpose
    W2m.2-3 plasmid. Contains U6-LacI-sgRNA A and is ppart of the CAMERA2m system for cellular recording in mammalian cells
    Depositor
    Insert
    U6-LacI-sgRNA A
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pWT060f

    Plasmid
    #107907
    Purpose
    W2m.1-2 plasmid. Contains U6-sgRNA D (CCR5) and is ppart of the CAMERA2m system for cellular recording in mammalian cells
    Depositor
    Insert
    U6-sgRNA D (CCR5)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pQdCas12a.luxR(mut)-sggfp(C)

    Plasmid
    #236043
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pQdCas12a.luxR(mut)-sggfp(B)

    Plasmid
    #236041
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pWT007h

    Plasmid
    #107892
    Purpose
    W1.0.1c plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA(con.) and is part of CAMERA 1.0.1c
    Depositor
    Insert
    PTetO-SD8-Cas9, PLac-sgRNA(con.)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pWT060j

    Plasmid
    #107906
    Purpose
    W2m.0-4 plasmid. Contains U6-sgRNA C (CCR5) and is ppart of the CAMERA2m system for cellular recording in mammalian cells
    Depositor
    Insert
    U6-sgRNA C (CCR5)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1801 - 1840 of 8502 results