We narrowed to 1,705 results for: SUA

1. HR180-LGR5-CreER

   Plasmid

   #129093

   Purpose

   KI CreER with a puromycin/mRuby selection cassette into human LGR5 locus

   Depositor

   Toshiro Sato

   Article

   Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

   Insert

   CreERT2 and LGR5 homology arms (LGR5 Human)
   

   Use

   Homologous recombination donor plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW-NbVHH05-GFP

   Plasmid

   #171570

   Purpose

   Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cells

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-GFP (UBE2J1 Human)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   fly actin5C promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-PM-TORCAR

   Plasmid

   #64930

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   Lyn-TORCAR (EIF4EBP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Frankenbody-FLAG-SNAP

   Plasmid

   #175295

   Purpose

   Track mature FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with SNAP-tag
   

   Use

   Tags

   SNAP-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Lamp1-YFP

   Plasmid

   #1816

   Purpose

   Mammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   lysosome associated membrane protein 1 (Lamp1 Rat)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   D50E (not important for function of plasmid)

   Promoter

   Available since

   Feb. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

   Plasmid

   #216125

   Purpose

   Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

   Plasmid

   #216124

   Purpose

   Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. hGFAP-GFP

   Plasmid

   #40592

   Depositor

   Albee Messing

   Article

   Zhuo et al Dev Biol. 1997 Jul 1;187(1):36-42.

   Insert

   GFP-S65T
   

   Use

   Mouse Targeting

   Tags

   MP-1 fragment (mouse protamine 1 intron and polya…

   Expression

   Bacterial and Mammalian

   Mutation

   Construct contains a mutated, humanized hGFP(S65T…

   Promoter

   hGFAP promoter

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBa.GFP-KIF16B tail 458-1317

   Plasmid

   #134624

   Purpose

   Mammalian expression of human KIF16B 458-1317

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF16B (KIF16B Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 457 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAW-NbVHH05-mCherry

    Plasmid

    #171572

    Purpose

    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-mCherry (UBE2J1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-DIO-IGK-dAPEX2-KDEL

    Plasmid

    #117183

    Purpose

    Plasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    IGK-dAPEX2-KDEL
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. alpha catenin conformation sensor

    Plasmid

    #71709

    Purpose

    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV5-25BoxB

    Plasmid

    #60817

    Purpose

    Cloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFP

    Depositor

    Maria Carmo-Fonseca

    Article

    Martin et al Cell Rep. 2013 Sep 26;4(6):1144-55. doi:

    Insert

    25x BoxB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    25 repeats of BoxB sequence

    Promoter

    CMV

    Available since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-GalT-mCherry

    Plasmid

    #214271

    Purpose

    Mammalian expression of GalT-mCherry (Golgi marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    GalT(1-82)-mCherry (B4GALT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. FKBP-Dronpa

    Plasmid

    #87709

    Purpose

    FKBP-tagged Dronpa.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    FKBP-Dronpa
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB.GFP-KIF1A tail 395-1698

    Plasmid

    #134618

    Purpose

    Mammalian expression of mouse KIF1A 395-1698

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 394 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBa.GFP-KIF1C tail 400-1103

    Plasmid

    #134621

    Purpose

    Mammalian expression of human KIF1C 400-1103

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 399 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

    Plasmid

    #134613

    Purpose

    Mammalian expression of rat KIF5C 378-955

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5C (Kif5c Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-TORCAR-NLS

    Plasmid

    #64931

    Purpose

    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR-NLS (EIF4EBP1 Synthetic, Human)
    

    Use

    Tags

    Cerulean, Nuclear localization signal (NLS), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Syn1-DIO-VGLUT2:miniSOG

    Plasmid

    #214200

    Purpose

    For Cre-dependent expression of VGLUT2:miniSOG (miniSOG is lumen-facing and HA-tagged)

    Depositor

    Thomas Hnasko

    Article

    Steinkellner et al ACS Chem Neurosci. 2021 Feb 17;12(4):626

    Insert

    Slc17a6 tagged with miniSOG and hemagglutinin
    

    Use

    AAV and Cre/Lox

    Tags

    miniSOG (tagged with HA) is fused to the first in…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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