We narrowed to 10,808 results for: T7
-
Plasmid#36049DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only
-
DJ-1 TEV site pET15b
Plasmid#60687PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationTEV site was inserted (see comments below).PromoterT7Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG8H-EWS_1-264
Plasmid#180467PurposeExpresses the construct His8-EWS_1-264 with a TEV cleavage site for the removal of the His8 tagDepositorAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-his-Gluc-iLID
Plasmid#172096PurposeExpresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteriaDepositorInsertGaussia Luciferase - iLID
ExpressionBacterialMutationGluc Mutations - M43L, M110LPromoterT7Available SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del1 pET28a
Plasmid#122115PurposeExpression Brg1 amino acids 1-381 in bacteriaDepositorAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Twitch-3 pRSETB
Plasmid#48204PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-3A
Tags6xHIS, cpVenusCD, and mCerulean3ExpressionBacterialPromoterT7Available SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
(367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)
Plasmid#168099PurposeHA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGADepositorInsertHA-nUbc-OGA(544-706)
TagsHA-tagExpressionMammalianMutationOGA truncated to contain residues 544-706PromoterT7/CMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains1
Plasmid#78461PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
TagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2149-2170; Q2513R (please see…PromoterT7Available SinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-NLP-cMyc LbaCas12a
Plasmid#182123PurposepET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteriaDepositorInsert2xNLS-NLP-cMyc LbaCas12a
Tags6xHisExpressionBacterialPromoterT7Available SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
WT N-His Ana GvpC in pET28a
Plasmid#153294PurposeExpresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (N-terminal his-tagged)
TagsHis-tagExpressionBacterialMutationAdded a hexahistidine tag after the methionine at…PromoterT7Available SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::hGluc
Plasmid#186768PurposeAn mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.DepositorInsertGaussia luciferase
PromoterT7Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-EKAR-EV
Plasmid#123339PurposeYellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.DepositorInsertVenus-cpVenus-FLARE-EKAR-EV
Tags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF714-pET28a-Hs-FL-METTL20-NHis wt
Plasmid#85115Purposeexpression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pF708-pET28a-Hs-delta38-METTL20-NHis wt
Plasmid#85109Purposeexpression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0004
Plasmid#42244PurposegRNA targeted to zebrafish gene fh [site #2]DepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pF711-pET28a-Hs-delta25-MCAD-NHis wt
Plasmid#85112Purposeexpression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet23a_RBPMS_FL
Plasmid#59392PurposePlasmid for bacterial overexpression of RBPMSDepositorAvailable SinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LOV-ipaA L514K L531E
Plasmid#40253DepositorInsertLOV-ipaA L514K L531E
Tags6xHisExpressionBacterialMutationL514K; L531E; Jα helix region hybridized (AsLOV2 …PromoterT7Available SinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP3-His
Plasmid#67866Purposebacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tagDepositorInsertMRPP3 (KIAA0391 Human)
TagsHisExpressionBacterialMutationAmino acid 46 of recombinant MRPP3 is preceded by…PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP3
Plasmid#129501PurposeExpresses AausFP3 constitutively in E. coli (most strains)DepositorInsertAausFP3
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only