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pCAG_Xph20-mRuby2-CCR5TC

Plasmid

#135531

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

mRuby2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBZCas13b-HEPN

Plasmid

#89901

Purpose

Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.

Depositor

Feng Zhang

Article

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

Cas13b

Expression

Bacterial

Mutation

R116A/H121A/R1177A/H1182A

Promoter

Lac

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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hDAGLa-V5

Plasmid

#87676

Purpose

Expresses human DAGL? protein with intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Article

Singh et al Biosci Rep. 2016 May 6;36(3). pii: e0033

Insert

Human Diacylglycerol Lipase Alpha (DAGLA Human)

Tags

V5 tag

Expression

Mammalian

Promoter

T7

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pActinin-miRFP703

Plasmid

#79992

Purpose

Alpha actinin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Alpha-Actinin (ACTN1 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

c

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pEERM6 Km

Plasmid

#64027

Purpose

Plasmid for integration into sqs site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNAIIIB rac1 QL

Plasmid

#61633

Purpose

mammalian expression of rac1 constitutively active mutant

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

Q61L, constitutively active

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pCDNAIIIB cdc42 WT

Plasmid

#61635

Purpose

mammalian expression of cdc42 wt

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

cdc42 (CDC42 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 Δ593-618

Plasmid

#47901

Purpose

Expresses myc tagged Miro2 lacking transmembrane domain

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 Δ593-618 (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

Δ593-618, lacks transmembrane domain. Also see De…

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 S430N

Plasmid

#47899

Purpose

Expresses myc tagged Miro2 S430N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 S430N (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

S430N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 R425V

Plasmid

#47898

Purpose

Expresses myc tagged Miro2 R425V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 R425V (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

R425V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pENTR SD Age HsSAS-6

Plasmid

#46381

Purpose

For expression of C-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Gateway Cloning

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1V5-His Snk/Plk2 D223N

Plasmid

#22218

Depositor

Wafik El-Deiry

Article

Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

Insert

Snk/Plk2 (PLK2 Human)

Expression

Mammalian

Mutation

single amino acid substitution mutant, aspartic a…

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
GFP-pCdc42

Plasmid

#23240

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Cdc42 (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pBabe hygro MTH1

Plasmid

#21296

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pLKO.1 shMTH1-1

Plasmid

#21297

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

shMTH1-1 (NUDT1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
HOXB7 (human) HIS-tag pET

Plasmid

#8538

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Tags

His and T7

Expression

Bacterial

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
PB_EF1a-DjCas13d-msfGFP-Blast

Plasmid

#226008

Purpose

DjCas13d protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Insert

DjCas13d RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-KDM6A

Plasmid

#203580

Purpose

Expresses wild-type KDM6A in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

Insert

KDM6A (KDM6A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ICUE4

Plasmid

#181846

Purpose

Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

ICUE4 (RAPGEF3 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

Plasmid

#181975

Purpose

Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)

Use

Lentiviral

Mutation

NGFR truncation: aa 11-276

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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