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pMal-SLFN2

Plasmid

#174322

Purpose

Bacterial expression of MBP-tagged mouse SLFN2

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

SLFN2 (Slfn2 Mouse)

Tags

MBP

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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hisSUMO-Gli1

Plasmid

#87225

Purpose

Express hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

GLI1 (Gli1 Mouse)

Tags

hisSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-R

Plasmid

#122345

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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GFP Snail 4SA

Plasmid

#16227

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

4SA: S108A, S112A, S116A, S120A

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pQTEV-AK1

Plasmid

#31290

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

adenylate kinase 1 (AK1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-FSF1

Plasmid

#110641

Purpose

Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

FSF1 (FSF1 Budding Yeast)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFN-HaloTag-TalinR8-SpyTag

Plasmid

#192045

Purpose

Plasmid expresses human talin R8 domain flanked between a HaloTag and a SpyTag

Depositor

Ionel Popa

Article

Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

Insert

TLN1 (TLN1 Human)

Tags

HaloTag and SpyTag

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFN-HaloTag-TalinR7R8-AviTag

Plasmid

#192047

Purpose

Plasmid expresses human talin R7R8 domain flanked between a HaloTag and a AviTag

Depositor

Ionel Popa

Article

Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

Insert

TLN1 (TLN1 Human)

Tags

AviTag and HaloTag

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a SVEM hphMX6 lys1-I-SceI cleavage site

Plasmid

#67648

Purpose

I-SceI cleavage site at lys1-hphMX6

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

lys1 (lys1 Fission Yeast)
hphMX6

Use

To test i-scei cleavage efficiency

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS315-48nt-telo-I-SceI

Plasmid

#67623

Purpose

telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

3' gal1 unique sequence
ura4 (ura4 Fission Yeast)
48 bp telomere repeats
hph

Use

Yeast cen/ars plasmid

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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GFP Snail 2A4E

Plasmid

#16231

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

2A4E: S97A, S101A, S108E, S112E, S116E, S120E

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pMflT-o4

Plasmid

#101312

Purpose

Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Insert

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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PGEX-DDX21-D2+C

Plasmid

#179243

Purpose

protein purification in E. coli

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21-D2+C (DDX21 Human)

Tags

GST-flag

Expression

Bacterial

Mutation

aa396-782

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Q842gp120-8his

Plasmid

#100932

Purpose

Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q842.d12) Env

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-EKAR

Plasmid

#181858

Purpose

Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-EKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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Flag Snail 4SE

Plasmid

#16223

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

Flag

Expression

Mammalian

Mutation

4SE: S108E, S112E, S116E, S120E

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
DT050A

Plasmid

#159411

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23101 promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1180 EACMCV DNA-B 1.6mer

Plasmid

#159137

Purpose

Infectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 2007 DNA-B

Depositor

Vincent Fondong

Article

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

ACMV DNA-B 1.5mer

Use

Infectious clone for bombarding plants

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-GNB2 5'-UTR

Plasmid

#115355

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of GNB2

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

GNB2 5'-UTR (GNB2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

Plasmid

#49218

Purpose

Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Retroviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Promoter

Viral LTR (or CMV)

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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