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M19 pCS-twhh-dsl-1myc

Plasmid

#17156

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

dorsalin-1 (GDF2 Chicken)

Tags

myc-tag and twhh promoter (D rerio)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pFbx15(-544/-527)-TK-Luc

Plasmid

#13414

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
syn3D27

Plasmid

#14676

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector; aa 1-27 …

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
L1-I219T

Plasmid

#15486

Depositor

Vance Lemmon

Article

Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

Insert

L1-I219T (L1CAM Human)

Expression

Bacterial

Mutation

Isoleucine 219 changed to Threonine

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
Syn3D27-146

Plasmid

#14678

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector; aa 27-14…

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only
Syn3M32A

Plasmid

#13399

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

Myc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. M32 of s…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pFbx15(-638/+114)-Luc

Plasmid

#13408

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-SNAP-p53DD

Plasmid

#244168

Purpose

Retroviral expression of SNAP-p53DD

Depositor

Kate Galloway

Article

Lende-Dorn et al Cell Rep. 2025 Sep 23;44(9):116226. doi:

Insert

SNAP-p53DD (Trp53 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-TagRFP657

Plasmid

#243767

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to TagRFP657. VHH-TagRFP657 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and TagRFP657-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-mNeptune2.5

Plasmid

#243765

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mNeptune2.5. VHH-mNeptune2.5 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mNeptune2.5-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-MS2-RecT-dCas9-BSD

Plasmid

#226108

Purpose

Using ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol 8 (2025). https://doi.org/10

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_PHF1_sgRNA

Plasmid

#246403

Purpose

Cas9/sgRNA expression plasmid targeting PHF1

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-mCardinal2

Plasmid

#243766

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCardinal2. VHH-mCardinal2 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mCardinal2-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-iRFP670

Plasmid

#243768

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to iRFP670. VHH-iRFP670 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and iRFP670-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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Empty(Ctrl)-eGFP-PGK-H2BRFP

Plasmid

#241864

Purpose

Control for epicenter-driven fluorescent reporters

Depositor

Elaine Fuchs

Article

Ge et al Cell. 2017 May 4;169(4):636-650.e14. doi

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Klf5-eGFP-PGK-H2BRFP

Plasmid

#241865

Purpose

Klf5 epicenter-driven fluorescent reporter

Depositor

Elaine Fuchs

Article

Ge et al Cell. 2017 May 4;169(4):636-650.e14. doi

Insert

Klf5-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSS22-IRES-NLS-eGFP

Plasmid

#241837

Purpose

Part I of the Donor construct for UBE3A reporter

Depositor

Albert Keung

Article

Kelkar et al Front Cell Dev Biol. 2025 Aug 29;13:1665

Insert

eGFP

Use

Donor plasmid

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-Merlin(dog)gRNA1

Plasmid

#214823

Purpose

A knockout vector for dog Merlin.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

A gRNA targeting the dog NF2(Merlin) gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-bleo-Merlin(dog)gRNA2

Plasmid

#214824

Purpose

A knockout vector for dog Merlin.

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

A gRNA targeting the dog NF2(Merlin) gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP703-eDHFR(69K6)-mSOS1-linkercat

Plasmid

#214827

Purpose

Encoding mSOS1-linkercat fused to miRFP703

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

miRFP703-eDHFR(69K6)-mSos1-linkercat

Tags

miRFP703

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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