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Showing: 921 - 940 of 2487 results

  1. HA-IFITM3-F8-TAG

    Plasmid
    #122475
    Purpose
    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon
    Promoter
    Available since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122475-plasmid-map-sequence-id-237716

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  2. pIN4_tsp-6_mScarlet

    Plasmid
    #182935
    Purpose
    Expresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)
    Depositor
    Maureen Barr
    Article
    Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6
    Insert
    Ptsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)
    Use
    Tags
    flexible linker (GGGGS)x3 and mScarlet tag
    Expression
    Worm
    Mutation
    Promoter
    tsp-6
    Available since
    Aug. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    182935-plasmid-map-sequence-id-368447

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  7. RGD-sfGFP-I27-CC

    Plasmid
    #85392
    Purpose
    MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    RGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85392-plasmid-map-sequence-id-172694

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  11. K15/LacZ

    Plasmid
    #44265
    Depositor
    George Cotsarelis
    Article
    Liu et al J Invest Dermatol. 2003 Nov;121(5):963-8
    Insert
    K15 promoter (-4.8) (Krt15 Mouse)
    Use
    Mouse Targeting; Β-galactosidase
    Tags
    LacZ
    Expression
    Mammalian
    Mutation
    Contains the murine K15 promoter (-4.8) fragment
    Promoter
    murine K15 promoter (-4.8) fragment
    Available since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pEF NS34a-msGFP-'Q-mito

    Plasmid
    #158764
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Article
    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158764-plasmid-map-sequence-id-312950

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  17. pFastBac HT JS-Munc18b

    Plasmid
    #135554
    Purpose
    Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.
    Depositor
    Jingshi Shen
    Article
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18b (Stxbp2 Rat)
    Use
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    March 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    135554-plasmid-map-sequence-id-272494

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  20. pPTG-GG-sg2+sg3

    Plasmid
    #127199
    Purpose
    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter
    Depositor
    Jordan Green
    Article
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Insert
    tRNA-gRNA
    Use
    Empty sgrna plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127199-plasmid-map-sequence-id-248025

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Showing: 921 - 940 of 2487 results