We narrowed to 4,743 results for: MLS

1. EGFP-uTEV1-CB5

   Plasmid

   #171007

   Purpose

   HiLITR protease with CB5 C-terminal tmd targeting information (ER)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-CB5(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDACR3882 [rab-3p::HYlight::let-858utr]

   Plasmid

   #207666

   Purpose

   Expresses worm-optimized HYlight in C. elegans neurons

   Depositor

   Daniel Colón-Ramos

   Article

   Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   HYlight Biosensor
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rab-3

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG496

   Plasmid

   #226750

   Purpose

   Expresses exogenous MTCH2

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MTCH2 (MTCH2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #106249

   Purpose

   Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

   Insert

   dead S. aureus Cas9 KRAB T2A PuroR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA-NLS and NLS-KRAB

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   hUbC

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Donor-PIGA

   Plasmid

   #153530

   Purpose

   Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   PIGA (a 1,991-bp fragment from intron 5 and exon 6)
   

   Use

   Donor plasmid

   Tags

   N/A

   Expression

   Mutation

   No mutation

   Promoter

   No promoter

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

   Plasmid

   #81185

   Purpose

   Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

   Depositor

   Jennifer Doudna

   Article

   Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

   Inserts

   Cas5c
   Cas8c
   Cas7
   

   Use

   CRISPR

   Tags

   6xHis - MBP - TEV protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PREX2 pcdna3.1 V5/his

   Plasmid

   #41555

   Depositor

   Ramon Parsons

   Article

   Fine et al Science. 2009 Sep 4;325(5945):1261-5.

   Insert

   PREX2a (PREX2 Human)
   

   Use

   Tags

   V5 and his

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

8. Tagged-IRES

   Plasmid

   #35570

   Depositor

   Marian Waterman

   Article

   Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

   Insert

   Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase and MS2 Stem Loop

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. Rap1A FLARE

   Plasmid

   #134926

   Purpose

   Rap1A biosensor

   Depositor

   Klaus Hahn

   Article

   O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

   Insert

   Rap1A, mCerulean3, RaIGDS RBD, YPet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rap1B FLARE

    Plasmid

    #134927

    Purpose

    Rap1B biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet, Cerulean, Rap1B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Cre-6xHis

    Plasmid

    #62939

    Purpose

    Expression of Cre-6xHis in bacterial of cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cre recombinase
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA24N-ligase-cTF

    Plasmid

    #87743

    Purpose

    IPTG-inducible expression of ligase-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and cTF (chimeric transcription factor base…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-H2B-Dendra

    Plasmid

    #140559

    Purpose

    Red to green photoconvertible chromosome marker

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    H2B
    

    Use

    Tags

    Dendra

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-REPORT(PGK/CMV)-GtwyA

    Plasmid

    #208574

    Purpose

    LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    mnucTomato
    HSVtk
    Neomycin
    d2nucEGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV and minP

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-mouse p19ARF-IRES-GFP

    Plasmid

    #107147

    Purpose

    Mammalian expression of mouse Arf

    Depositor

    Martine Roussel

    Article

    Kuo et al Cancer Res. 2003 Mar 1;63(5):1046-53.

    Insert

    Arf [alternative reading frame] (Cdkn2a Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RalGDS-YPet

    Plasmid

    #134929

    Purpose

    Fluorescently tagged affinity reagent

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. DT049A

    Plasmid

    #159410

    Purpose

    Constitutive murine mature BDNF expression, controlled under E.coli tet promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tet promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-uTEV1-NES

    Plasmid

    #171006

    Purpose

    HiLITR protease with C-terminal NNES (Cytoplasmic)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-NES
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM5082

    Plasmid

    #85121

    Purpose

    PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    PkaiB-KaiC-ECFP
    

    Use

    Tags

    ECFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM5085

    Plasmid

    #85122

    Purpose

    PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
    

    Use

    Tags

    ECFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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