We narrowed to 9,775 results for: Tec;

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pGFP-PGL-1-mHP1alpha

Plasmid

#181899

Purpose

Fluorescently tagged HP1alpha fused to PGL-1

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PGL-1-mHP1alpha (Cbx5 Mouse, Nematode)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-Truncated-Kir3.2-StreptagII

Plasmid

#177263

Purpose

Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

Depositor

Arthur Laganowsky

Article

Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

Insert

G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)

Tags

StreptagII

Expression

Insect

Mutation

Residues 49-378

Promoter

polyhedrin promoter

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pIRESneo2 mycBioID-Tollip M240A,F241A

Plasmid

#178055

Purpose

myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

mycBioID

Expression

Mammalian

Mutation

Changed Methionine 240 to Alanine and changed Phe…

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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str-KDEL_Stx5S-SBP-mCitrine

Plasmid

#154845

Purpose

Synchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Stx5 (Short isoform) (STX5 Human)

Tags

Streptavidin Binding Protein (SBP) and mCitrine

Expression

Mammalian

Mutation

Lacks amino acids 1-54, enocdes short isoform of …

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m5~451-CMV2-mir1

Plasmid

#164679

Purpose

Express miR-144(mutant5)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant5)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m4~451-CMV2-mir1

Plasmid

#164678

Purpose

Express miR-144(mutant4)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant4)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m3~451-CMV2-mir1

Plasmid

#164677

Purpose

Express miR-144(mutant3)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant3)~451 (MIR144 Human)
mir-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m2~451-CMV2-mir1

Plasmid

#164676

Purpose

Express miR-144(mutant2)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant2)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m1~451-CMV2-mir1

Plasmid

#164675

Purpose

Express miR-144(mutant1)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant1)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir125~451-CMV2-mir1

Plasmid

#164669

Purpose

Express miR-125a~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-125a~451 (MIR125A Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP N1 AURKA 1-110

Plasmid

#157755

Purpose

Expression of AURKA Nter (aa1-110):GFP in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Mammalian

Mutation

The first 110 amino acids of AURKA were cloned

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

Plasmid

#123336

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-cpCerulean3-FLARE-AKAR

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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Opie2-PuroR[dsxM]

Plasmid

#131616

Purpose

Expresses a functional PuroR only in males and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR[dsxM] (dsx Fly)
dsRed

Expression

Insect

Promoter

Opie2 and hr5Ie1

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

Plasmid

#124066

Purpose

E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-R232Q-sfGFP

Tags

sfGFP

Expression

Bacterial

Mutation

R232Q

Promoter

trc

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP2B-siRNAres

Plasmid

#115333

Purpose

expresses siRNA-resistant mCherry-CHMP2B in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

mutated to be resistant to siRNA knockdown

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-ARL4D-no stop

Plasmid

#67337

Purpose

hArl4D without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pmCherry_C1 I3(resist)

Plasmid

#61399

Purpose

Expresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

mCherry

Expression

Mammalian

Mutation

silent mutations of bases C183T T192C T189C

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pSEW-Venus-Sec16A-deltaN

Plasmid

#66605

Purpose

Lentiviral vector for express truncated form of Sec16A as a fusion with Venus FP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec16A (SEC16A Human)

Tags

Venus (EYFP derivative)

Expression

Mammalian

Mutation

encodes amino acids 1107-1217 of human Sec16A

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestMYC_RBPMS_KDR

Plasmid

#65667

Purpose

MYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

MYC

Expression

Mammalian

Mutation

introduced HindIII site to exchange FLAG-HA tag w…

Promoter

CMV, 2x Tet

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_R38Q

Plasmid

#65094

Purpose

Destination vector with RBPMS with R38Q mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

changed arginine 38 to glutamine

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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