We narrowed to 13,693 results for: crispr cas9

1. pZNF134.1.0-gDNA

   Plasmid

   #112416

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF134

   Depositor

   Kevin White

   Insert

   ZNF134 (ZNF134 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNR1D2.1.0-gDNA

   Plasmid

   #112410

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR1D2

   Depositor

   Kevin White

   Insert

   NR1D2 (NR1D2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZNF354B.1.0-gDNA

   Plasmid

   #112405

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF354B

   Depositor

   Kevin White

   Insert

   ZNF354B (ZNF354B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZNF75A.1.0-gDNA

   Plasmid

   #112403

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF75A

   Depositor

   Kevin White

   Insert

   ZNF75A (ZNF75A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HCP8

   Plasmid

   #166110

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5

   Depositor

   Matthias Heinemann

   Insert

   Whi5-sg106 (WHI5 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRPCE

   Plasmid

   #193712

   Purpose

   Expresses cytosine deaminase linked deactivated-Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection

   Depositor

   Martin Buck

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV-XAF1

   Plasmid

   #140290

   Purpose

   To knockout murine XAF1 gene by CRISPR-Cas9 system

   Depositor

   Adriano Aguzzi

   Insert

   XAF1 sgRNA (Xaf1 Mouse)
   

   Use

   Lentiviral

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tet-inducible Luciferase reporter

   Plasmid

   #64127

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   TetOx13-CMV

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-hRad51

   Plasmid

   #125570

   Purpose

   Expresses the Rad51 protein in mammalian cells under CMV promoter

   Depositor

   David Liu

   Article

   Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

   Insert

   Rad51 (RAD51 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GAL4UAS-Luciferase reporter

    Plasmid

    #64125

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    GAL4UAS
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GAL4UAS

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG ERT2-Cre-ERT2 lox

    Plasmid

    #183447

    Purpose

    CreOFF tamoxifen-inducible Cre

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    ERT2-Cre-ERT2
    

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Tet-inducible mCherry reporter

    Plasmid

    #64128

    Purpose

    Photoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    TetOx13-CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCB591

    Plasmid

    #141096

    Purpose

    E. coli-Lactobacilli shuttle vector for recombineering template cloning

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-Rad18UBD

    Plasmid

    #119005

    Purpose

    Mammalian expression of the human Rad18 Ubiquitin binding domain and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Rad18
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-RNF169UBD

    Plasmid

    #119006

    Purpose

    Mammalian expression of the human RNF169 Ubiquitin binding domain and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    RNF169 (RNF169 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCN-EF2132tet

    Plasmid

    #107191

    Purpose

    Expresses EF2132 recombinase for performing recombineering in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    EF2132
    tetR
    

    Expression

    Bacterial

    Promoter

    p23

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUPRT::DHFR-D

    Plasmid

    #58528

    Purpose

    replace TgUPRT with DHFR by homologous recombination

    Depositor

    David Sibley

    Article

    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

    Insert

    DHFR cassette
    

    Use

    Gateway cloning

    Mutation

    pyrimethamine resistant

    Promoter

    DHFR

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hRad51(K133R)

    Plasmid

    #125571

    Purpose

    Expresses the hRad51(K133R) protein in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51 (RAD51 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    K133R

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAVS1_sgRNA

    Plasmid

    #100554

    Purpose

    Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    AAVS1 sgRNA
    

    Promoter

    U6

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid

    #174137

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge