We narrowed to 8,752 results for: App;

1. pJK509

   Plasmid

   #71599

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn mutant (AaPurE1fww-H59N)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, histidine…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK021

   Plasmid

   #75060

   Purpose

   Produces Saccharomyces cerevisiae ADE2 (N5-carboxyaminoimidazole ribonucleotide mutase and N5-carboxyaminoimidazole ribonucleotide synthetase fusion protein)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Insert

   phosphoribosylaminoimidazole carboxylase ADE2 (ADE2 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E. coli optimized codons in first 25 residues

   Promoter

   lac

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK642

   Plasmid

   #72391

   Purpose

   Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)

   Depositor

   T. J. Kappock

   Insert

   glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutated tyrosine-397 to phenylalanine to block ad…

   Promoter

   T7

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57 moxDendra2

   Plasmid

   #89791

   Purpose

   bacterial expression of moxDendra2

   Depositor

   Erik Snapp

   Article

   Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

   Insert

   moxDendra2
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDE43-MCSq17

   Plasmid

   #27333

   Depositor

   Dirk Schnappinger

   Article

   Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

   Insert

   qtag17
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   Available since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK650

   Plasmid

   #72400

   Purpose

   Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)

   Depositor

   T. J. Kappock

   Insert

   glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutated tyrosine-397 to phenylalanine to block ad…

   Promoter

   T7

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJBL002

   Plasmid

   #71202

   Purpose

   Antisense Control Plasmid

   Depositor

   Julius Lucks

   Article

   Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK122

   Plasmid

   #74295

   Purpose

   Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusion

   Depositor

   T. J. Kappock

   Insert

   hypothetical acetic acid resistance protein B
   

   Use

   Tags

   NusA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDE43-MCSq22

   Plasmid

   #27335

   Depositor

   Dirk Schnappinger

   Article

   Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

   Insert

   qtag22
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   Available since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK237

    Plasmid

    #73339

    Purpose

    Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion

    Depositor

    T. J. Kappock

    Insert

    citrate synthase
    

    Use

    Tags

    His6, Ssp intein, and chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDE43-MCKq20

    Plasmid

    #27334

    Depositor

    Dirk Schnappinger

    Article

    Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

    Insert

    qtag20
    

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDE43-MCKq19

    Plasmid

    #27286

    Depositor

    Dirk Schnappinger

    Article

    Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

    Insert

    qtag19
    

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDE43-MCKq26

    Plasmid

    #27336

    Depositor

    Dirk Schnappinger

    Article

    Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

    Insert

    qtag26
    

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK103

    Plasmid

    #73265

    Purpose

    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)

    Depositor

    T. J. Kappock

    Insert

    hypothetical acetic acid resistance protein B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK610

    Plasmid

    #72531

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK540

    Plasmid

    #72446

    Purpose

    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK552

    Plasmid

    #72451

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)

    Depositor

    T. J. Kappock

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Val319 to Phe and inserted Val2

    Promoter

    T7

    Available since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK141

    Plasmid

    #73316

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    His6, Pas2p signal sequence, and c-myc

    Expression

    Yeast

    Mutation

    synthetic gene

    Promoter

    AOX1

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK586

    Plasmid

    #72459

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes tryptophan-34 to phenylalanine, …

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK565

    Plasmid

    #72454

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Ala/Trp165>Phe mutant (AaPurE1fwf-H59A)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes tryptophan-34 to phenylalanine, …

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge