We narrowed to 34,877 results for: CaS

1. pMBP-AaCas12b

   Plasmid

   #113433

   Purpose

   Expresses Expresses 10xHis-MBP fused to AaCas12b

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AaCas12b
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. BB3cN_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104906

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Mammalian_SpCas9_Target-AID2S

   Plasmid

   #188648

   Purpose

   Expresses SpCas9_Target-AID2S in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID2S-UGI
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SpCas9

   Promoter

   pCMV

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SUMO_TwinStrep_Cas7-11_crRNA

   Plasmid

   #197612

   Purpose

   For protein purification of Cas7-11

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   SUMO_TwinStrep_Cas7-11 with crRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA-SpCas9-g5

   Plasmid

   #80788

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AsdCas12a VPR

   Plasmid

   #188512

   Purpose

   Expresses FLAG tagged AsdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D908A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV_Efs_hSpCas9_NLS_FLAG-SV40

   Plasmid

   #97307

   Purpose

   AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   AAV and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223-CASK

   Plasmid

   #23470

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   CASK (CASK Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pMA-SpCas9-g6

   Plasmid

   #80789

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-EF1adCas9VP64T2ABsd-W

    Plasmid

    #112919

    Purpose

    Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9VP64-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459_gRNA-AAVS1_hspCas9

    Plasmid

    #193309

    Purpose

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LbdCas12a VPR

    Plasmid

    #188513

    Purpose

    Expresses FLAG tagged LbdCas12a VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_Caspase-4_K356D

    Plasmid

    #214312

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    K356D

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDNR-SpCas9

    Plasmid

    #80425

    Purpose

    Used for in vitro transcription of SpCas9 mRNA.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    none

    Expression

    Mutation

    codon optimized for human

    Promoter

    T7

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-T3-CjCAS9-pA

    Plasmid

    #131543

    Purpose

    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    FLAG-SV40NLS, SV40NLS, and polyA

    Expression

    Mammalian

    Mutation

    Human codon-optimized

    Promoter

    CAG, T3

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas12j-8-puro

    Plasmid

    #194966

    Purpose

    The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

    Depositor

    Yongming Wang

    Article

    Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Mammalian_SpCas9_Target-AID3S

    Plasmid

    #188649

    Purpose

    Expresses SpCas9_Target-AID3S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID3S-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SETMAR-Cas9

    Plasmid

    #186701

    Purpose

    Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETMAR-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS and P2A

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1_Cas9-hGem_gRNA2

    Plasmid

    #217659

    Purpose

    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA2 targeting the human AAVS1 safe harbour locus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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