We narrowed to 1,917 results for: FRT

1. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)

   Plasmid

   #204699

   Purpose

   This plasmid allows for inducible expression of engineered BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-p300 (BRD4, EP300 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   deletion of all TF-bidning domains in p300

   Promoter

   CMV

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(H*IT)

   Plasmid

   #204698

   Purpose

   This plasmid allows for inducible expression of engineered BRD4-p300(H*IT) fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-p300(H*IT) (BRD4, EP300 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   D1399Y substitution in p300

   Promoter

   CMV

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT-ChemoG-NAD-NLS3x

   Plasmid

   #193822

   Purpose

   CMV overexpression of ChemoG-NAD in the nucleus of mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG-NAD-NLS3x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K, T225R; HT7: L271E

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5/FRT/TO-ChemoL-NAD-NLS3x

   Plasmid

   #193828

   Purpose

   CMV overexpression of ChemoL-NAD in the nucleus of mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoL-NAD-NLS3x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K, T225R; HT7: L271E

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT/TO-[4xCox8]-ChemoL-NAD

   Plasmid

   #193829

   Purpose

   CMV overexpression of ChemoL-NAD in the mitochondria of mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   [4xCox8]-ChemoL-NAD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K, T225R; HT7: L271E

   Promoter

   CMV

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO_[Cox8a]x2_STf

   Plasmid

   #182003

   Purpose

   CMV overexpression of SNAP-tag fast in the mitochondrial matrix of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   [Cox8a]x2_STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p{Mhc-F4-678-DsRed::FRT-RC,attB}

   Plasmid

   #182187

   Purpose

   fluorescent reporter gene with yeast recombination site within intron

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   DsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p{Mhc-F4-678-DsRed::FRT,attB}

   Plasmid

   #182186

   Purpose

   fluorescent reporter gene with yeast recombination site within intron

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   DsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5 FRT-TO-EGFP-UPF1-R615A

   Plasmid

   #136001

   Purpose

   DOX-inducible R615A UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R615A

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5 FRT-TO-EGFP-UPF1-DEAA

    Plasmid

    #136002

    Purpose

    DOX-inducible DEAA UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    DE647AA

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TetO-Snu114-fSNAPc

    Plasmid

    #106989

    Purpose

    open reading frame of Snu114 inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    Snu114
    

    Use

    Tags

    fSNAP

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW707_pCAG-PV3-FRT-loxP-mCherry

    Plasmid

    #87571

    Purpose

    PV3 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW705_pCAG-PV1-FRT-lox2272-STOP

    Plasmid

    #87569

    Purpose

    PV1 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    Neomycin Resistance Gene
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5 FRT TO myc Separase delta 55

    Plasmid

    #59824

    Purpose

    Allows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF5/FRT-DEST EVC2ala7-Flag

    Plasmid

    #41010

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1189-1192 to Alanine

    Promoter

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pEF5/FRT-DEST EVC2ala5-Flag

    Plasmid

    #41009

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1185-1188 to Alanine

    Promoter

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5/FRT/TO-Flag-Tipin-L195A

    Plasmid

    #23118

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed Leucine 195 to Alanine

    Promoter

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 WT minigene

    Plasmid

    #169273

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 1-6 WT minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 Exon1-6 WT minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO - RBM28 Exon1-6 E5 GT>A minigene

    Plasmid

    #169274

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 1-6 GT>A minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 Exon1-6 GT>A minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NM_018077.2:c.(541+1_541+2delinsA)

    Promoter

    CMV

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

    Plasmid

    #226517

    Purpose

    Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    CalR-SNAP-tag2-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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