We narrowed to 14,955 results for: RING

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CALID

Plasmid

#169708

Purpose

Cre-activated light-inducible Dre recombinase

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_C2616

Plasmid

#91090

Purpose

Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

At7SL

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJY-RpABE-PDS3_IMS

Plasmid

#112875

Purpose

binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA targeting PDS3

Use

CRISPR

Expression

Plant

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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All-in-one split-ADAR2(E488Q, N496F)

Plasmid

#170148

Purpose

AAV vector carrying ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC(E488Q, N496F)-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pC012 LwCas13a locus with nontargeting spacer

Plasmid

#91904

Purpose

LwCas13a locus into pACYC184 with nontargeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a with non-targeting spacer

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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DMP1-GFPtopaz

Plasmid

#114270

Purpose

GFP labeling of mature osteoblasts and osteocytes.

Depositor

David Rowe

Article

Kalajzic et al Bone. 2004 Jul;35(1):74-82. doi: 10.1016

Insert

Dmp1 + GFPtopaz (Dmp1 Aequorea victoria, Mouse)

Expression

Mammalian

Promoter

Dmp1

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean-LaminB1-10

Plasmid

#55380

Purpose

Localization: Nuclear Envelope, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

LaminB1 (LMNB1 Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-Htr5b(3.7)-EGFP-sv40

Plasmid

#184411

Purpose

AAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

Depositor

Marylka Yoe Uusisaari

Article

Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

Insert

Mouse Htr5b gene promoter-EGFP (Htr5b Mouse)

Use

AAV

Tags

EGFP

Expression

Mammalian

Promoter

Mouse Htr5b gene

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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tsPurple chromoprotein promoter-less

Plasmid

#118007

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAG657 H2B-pFAST

Plasmid

#172862

Purpose

Expresses pFAST fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

H2B-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pME-CreLite

Plasmid

#131782

Purpose

Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

Cre/Lox; Gateway cloning

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCSDest2-NmeCas9-NLS-3XHA-NLS

Plasmid

#87448

Purpose

Mammalian expression of wild type Nme Cas9

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Nme Cas9

Use

CRISPR

Tags

3XHA, SV40 NLS, and cMyc-like NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag OSM

Plasmid

#12191

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159815

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-VA-NG

Plasmid

#220896

Purpose

Express haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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ZIKV_Trigger_27B

Plasmid

#75008

Purpose

Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_27B

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

Plasmid

#128404

Purpose

Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Exotoxin A (toxA P. aeruginosa)

Tags

6xHis tag and DsbA signal sequence for periplasmi…

Expression

Bacterial

Mutation

242DNNNS246 and 384DQNRT388 engineered glycosylat…

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-MEKK3

Plasmid

#12189

Depositor

Gary Johnson

Article

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

MEKK3 (Map3k3 Mouse)

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
LB124 (Bcl-2 from -1287 to -3934)

Plasmid

#15240

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -3934 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
PAX6 CKO 2/7 Donor plasmid

Plasmid

#68467

Purpose

donor plasmid for targeting PAX6 locus

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

FRT flanked exon4 of PAX6 gene (PAX6 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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