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pRL-TK let7 A

Plasmid

#11324

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation A from figure 4A of paper.

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
CRY2(L348F)-CreN

Plasmid

#75368

Purpose

Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Cre recombinase, residues 19-104

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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hPDI1_18-508_WT_pFLAG-CMV1

Plasmid

#31382

Depositor

David Ron

Article

Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

Insert

hPDI1 (P4HB Human)

Tags

FLAG M2

Expression

Mammalian

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

Plasmid

#35504

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-mE47

Plasmid

#34585

Depositor

Celeste Nelson

Article

Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

Insert

E47 (Tcf3 Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pGL2-Mcl-1

Plasmid

#19132

Depositor

Wafik El-Deiry

Article

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pTara:500

Plasmid

#60717

Purpose

Expresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORF

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

T7 RNAP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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CMV-myctagged MyoD

Plasmid

#8399

Depositor

Andrew Lassar

Insert

MyoD (Myod1 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pLenti_CamKIIa_GFP

Plasmid

#96941

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Ricardo Dolmetsch, Oleksandr Shcheglovitov

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

Green fluorescent protein

Use

Lentiviral

Promoter

CaMKIIa

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDonor_hU6

Plasmid

#69312

Purpose

sgRNA scaffold and human U6 promoter

Depositor

Andrea Ventura

Article

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

hU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

human U6 promoter

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDS220

Plasmid

#34980

Purpose

Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb–mCherry

Expression

Yeast

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pTEC27

Plasmid

#30182

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

tdTomato

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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7TC

Plasmid

#24315

Depositor

Roel Nusse

Article

ten Berge et al Cell Stem Cell. 2008 Nov 6. 3(5):508-18.

Insert

7xTcf-mCherry

Use

Lentiviral

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-ymNeongreen-SpHis5

Plasmid

#125704

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Tags

ymNeonGreen

Expression

Yeast

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3_UnaG-Flag-Sec61B

Plasmid

#83413

Purpose

Expressed N-term tagged UnaG on Sec61B

Depositor

Hyun-Woo Rhee

Article

Park et al ACS Chem Biol. 2016 May 27.

Insert

UnaG-Sec61B (SEC61B Human, Synthetic)

Expression

Mammalian

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-RSV-tdTomato-2A-GCaMP6s

Plasmid

#80316

Purpose

Fluorescent reporter for calcium signaling

Depositor

Darrell Kotton

Article

Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

Inserts

tdTomato
GCaMP6s

Use

Lentiviral

Promoter

RSV

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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LeGO-iCer2

Plasmid

#27346

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-Cerulean

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pSKI074

Plasmid

#11571

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Expression

Plant

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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A547 (miR-146a promoter)

Plasmid

#15091

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

miR-146a promoter

Expression

Mammalian

Available Since

June 6, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H-Ras_V12

Plasmid

#39504

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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