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Showing: 2401 - 2450 of 8038 results

  3. pAM71

    Plasmid
    #227621
    Purpose
    pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)
    Depositor
    Andreas Martin
    Article
    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    His-Prescission
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 24-160-GGSGSG-192-221
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227621-plasmid-map-sequence-id-455206

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  4. pME tdTomato-NLS

    Plasmid
    #135205
    Purpose
    Red fluorescent tag
    Depositor
    David Tobin
    Insert
    tdTomato
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    135205-plasmid-map-sequence-id-267013

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  6. pETSUMO2_Bacteroidetes-bGSDM

    Plasmid
    #212124
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Bacteroidetes bGSDM
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212124-plasmid-map-sequence-id-419109

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  10. pPV477

    Plasmid
    #42930
    Depositor
    James Lok
    Insert
    attL1::egfp (870 bp)::attL2
    Use
    Multi-site gateway donor plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    42930-plasmid-map-sequence-id-58193

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  11. pE-mApple L5L2

    Plasmid
    #187690
    Purpose
    C-Term Gateway cloning tag
    Depositor
    Mary Beckerle
    Insert
    mApple
    Use
    Gateway entry vector
    Tags
    mApple
    Expression
    Mutation
    WT
    Promoter
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    187690-plasmid-map-sequence-id-371407

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  14. pETSUMO2_Rhodoplanes-bGSDM

    Plasmid
    #212129
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Rhodoplanes bGSDM
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212129-plasmid-map-sequence-id-419110

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  15. pETSUMO2_Vitiosangium-bGSDM

    Plasmid
    #212127
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Vitiosangium bGSDM
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212127-plasmid-map-sequence-id-419098

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  17. pETSUMO2_Bradyrhizobium-bGSDM

    Plasmid
    #212126
    Purpose
    For expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Science. 2022 Jan 14;375(6577):221-225.
    Insert
    Bradyrhizobium bGSDM
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    Jan. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    212126-plasmid-map-sequence-id-418775

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  19. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid
    #58993
    Purpose
    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter
    Depositor
    Snorri Thorgeirsson
    Article
    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31
    Inserts
    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    EcoRV site in MCS converted to BamHI site
    Promoter
    CMV enhancer-chicken beta actin
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    58993-plasmid-map-sequence-id-457463

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  24. pB2H6

    Plasmid
    #163835
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163835-plasmid-map-sequence-id-337603

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  27. pB2H6*

    Plasmid
    #163836
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1 (R459M)
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    SHP1 R459M
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163836-plasmid-map-sequence-id-337604

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  28. zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)

    Plasmid
    #72638
    Purpose
    Tol2 based UAS modified with a CCR5TC binding site upstream of it, driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    72638-plasmid-map-sequence-id-445453

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  32. pENT-L1-Kozak-PH-domain-L2

    Plasmid
    #186354
    Purpose
    Entry clone with ORF encoding PH domain of human phospholipase C delta 1 flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Insert
    Pleckstrin Homology domain of PLCD1 (PLCD1 Synthetic, Human)
    Use
    Protein expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186354-plasmid-map-sequence-id-373422

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  33. pBMN DHFR-HiBiT / Lyt2

    Plasmid
    #179731
    Purpose
    Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites
    Depositor
    James Inglese
    Article
    Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23
    Insert
    human DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)
    Use
    Retroviral
    Tags
    HiBiT
    Expression
    Mutation
    Promoter
    LTR/MMLV
    Available since
    Oct. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    179731-plasmid-map-sequence-id-361671

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  34. p5E-elavl3

    Plasmid
    #72640
    Purpose
    Gateway p5E 5'entry clone with elavl3 (HuC) enhancer/promoter for pan-neuronal expression in zebrafish
    Depositor
    Joshua Bonkowsky
    Insert
    elavl3 enhancer (elavl3 Zebrafish)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72640-plasmid-map-sequence-id-141850

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  45. pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)

    Plasmid
    #72639
    Purpose
    Tol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
    Use
    Gateway
    Tags
    Expression
    Mutation
    Stop codon after mCherry- SEE DEPOSITOR COMMENTS …
    Promoter
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72639-plasmid-map-sequence-id-429021

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  48. CD55-Delta3

    Plasmid
    #220872
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-3
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-3, short consensus repeat (SCR) domains 1, …
    Promoter
    CMV
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220872-plasmid-map-sequence-id-443043

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Showing: 2401 - 2450 of 8038 results