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pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

Plasmid

#171428

Purpose

Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

FKBP-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGEN-dPspCas13b-NES-mCherry

Plasmid

#233609

Purpose

Expression of catalytically-inactive PspCas13b in mammalian cells

Depositor

Shintaro Iwasaki

Article

Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

Insert

dPspCas13b-NES-mCherry

Use

CRISPR

Tags

3xFlag and mCherry

Mutation

H133A/H1058A

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(Q61L)

Plasmid

#13720

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(Q61L) (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-FRB

Plasmid

#171427

Purpose

Expression of HaloTag N58-(GGS)X6-FRB in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-FRB

Expression

Mammalian

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TAC-ERP2

Plasmid

#80478

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(T17N)

Plasmid

#13721

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(T17N) (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Plasmid

#64512

Purpose

Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XmTagBFP2

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PKM2

Plasmid

#64698

Purpose

Expression of GFP tagged PKM2 in mammalian cells

Depositor

Axel Ullrich

Article

Stetak et al Cancer Res. 2007 Feb 15;67(4):1602-8.

Insert

PKM2 (PKM Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-PML IV/pRK5

Plasmid

#59742

Purpose

Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

Depositor

Xiaolu Yang

Article

Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

Insert

PML isoform IV (PML Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pLSU4cg10

Plasmid

#126078

Purpose

Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

longCaMV35S

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138133

Purpose

Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pPBCAG-rtTAM2-IH

Plasmid

#140827

Purpose

Expresses rtTAM2-IRES-HygR in mammalian cells

Depositor

Hideyuki Okano

Article

Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

Inserts

rtTAM2
HygR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-cMyc

Plasmid

#19772

Purpose

Non-integrating (episomal) expression of mouse c-Myc

Depositor

Shinya Yamanaka

Article

Okita et al Science. 2008 Nov 7. 322(5903):949-53.

Insert

c-Myc (Myc Mouse)

Expression

Mammalian

Available Since

Jan. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138130

Purpose

Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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hJAM-A pcDNA3.1

Plasmid

#70073

Purpose

Expresses human junctional adhesion molecule-A in mammalian cells

Depositor

Terence Dermody

Article

Danthi et al J Virol. 2006 Feb;80(3):1261-70.

Insert

junctional adhesion molecule-A (F11R Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138131

Purpose

Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138132

Purpose

Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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hSyn1 HRP-TM

Plasmid

#231500

Purpose

Expressing HRP on the cell surface of neurons

Depositor

Baljit Khakh

Article

Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

Insert

HRP

Use

AAV

Tags

Igk, HA, Myc, PDGFR-beta transmembrane domain

Expression

Mammalian

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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CD3Zeta-GFP

Plasmid

#38298

Depositor

Matthew Krummel

Article

Krummel et al Science. 2000 Aug 25;289(5483):1349-52.

Insert

CD3Zeta (Cd247 Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pLenti_EF1a_TauFRET_3xKQ_wpre

Plasmid

#207522

Purpose

Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

Depositor

Bradley Hyman

Article

Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

Insert

TauFRET_P301L_3xKQ

Use

Lentiviral

Tags

NeonGreen and mTurquoise2

Mutation

P301L; K311Q; K317Q; K321Q

Promoter

EF1a

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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