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S9_attL1-EGFP-C-term-attL2

Plasmid

#128473

Purpose

EGFP entry clone

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

EGFP entry clone

Expression

Bacterial

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pACYC-pVhb-vfFadBA

Plasmid

#215650

Purpose

pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)

Expression

Bacterial

Promoter

Vhb

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCmR2

Plasmid

#59015

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I alpha

Plasmid

#22154

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-4T1 human TBC1D7 (19-293)

Plasmid

#132617

Purpose

expresses human TBC1D7 (19-293) in E. coli

Depositor

Wenqing Xu

Article

Qin et al J Biol Chem. 2016 Apr 15;291(16):8591-60

Insert

TBC1D7 (19-293)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pT7-Venus-Emi1 (914)

Plasmid

#39854

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

Insert

Emi1 (FBXO5 Human)

Use

In vitro transcription

Tags

Venus

Promoter

T7

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-GLICNot-C-His

Plasmid

#140187

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

6xHis and GST

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-PAmCherry1_160-236-MCS

Plasmid

#60546

Purpose

Cloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 C-terminal fragment

Use

Cloning

Mutation

amino acids 160-236

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-PAmCherry1_1-159-MCS

Plasmid

#60545

Purpose

Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 N-terminal fragment

Use

Cloning

Mutation

amino acids 1-159

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NLS2D

Plasmid

#13285

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa150-aa155

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pLKO.1 EB1 shRNA #3

Plasmid

#37927

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 shRNA #3 (MAPRE1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

human U6

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCS4

Plasmid

#55751

Purpose

Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Expression

Bacterial

Mutation

Residues 1-59 of KanR

Promoter

T7-lac

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pET21c-IF3mt

Plasmid

#31078

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Ami…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
GST-RP2

Plasmid

#86072

Purpose

A bacterial expression plasmid encoding retinitis pigmentosa 2 (RP2) with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

RP2 (RP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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Integrin alpha5(1-623)-AHCys

Plasmid

#27301

Depositor

Timothy Springer

Article

Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

Insert

integrin alpha 5 (ITGA5 Human)

Expression

Mammalian

Mutation

alpha 5(1-623)+GG+TEV+GGEN+AHCys

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only
pET16b-Trx C35S

Plasmid

#36947

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Thioredoxin-1 C35S mutation (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

cys 35 to serine

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Flag-Timeless

Plasmid

#22892

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Timeless (TIMELESS Human)

Tags

Flag and His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pTP443

Plasmid

#216361

Purpose

xNup98-Nb1t (+1Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup98-Nb1t (+1Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMJ915v2+Nterm Spe1 +Cterm Age1 site

Plasmid

#88914

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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