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We narrowed to 18,460 results for: ENA

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Showing: 201 - 250 of 18460 results

  2. pPD1R

    Plasmid
    #86969
    Purpose
    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH
    Promoter
    T7 promoter
    Available Since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    86969-plasmid-map-sequence-id-173876

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  3. pfMDH_Triple

    Plasmid
    #67733
    Purpose
    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine, arginine 373 to…
    Promoter
    T7
    Available Since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pFnCpf1GG

    Plasmid
    #131010
    Purpose
    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of FnCas12a
    GFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131010-plasmid-map-sequence-id-257701

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  5. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

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  6. pCAP03-ermE*p

    Plasmid
    #120234
    Purpose
    Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.
    Depositor
    Bradley Moore
    Article
    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-
    Insert
    promoter cassette containing ermE*p promoter and ampicillin resistance gene
    Use
    Synthetic Biology
    Mutation
    bla (amp resistance gene)
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    120234-plasmid-map-sequence-id-233748

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  9. pLsCas13aGG

    Plasmid
    #131012
    Purpose
    Backbone plasmid for generating CRISPR arrays for LsCas13a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of LsCas13a
    GFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131012-plasmid-map-sequence-id-258107

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  10. pAsCpf1GG

    Plasmid
    #131011
    Purpose
    Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of AsCas12a
    GFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131011-plasmid-map-sequence-id-258106

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  11. pPD3C

    Plasmid
    #86971
    Purpose
    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 fused to phosphite dehydrogenase and P450cam
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86971-plasmid-map-sequence-id-173928

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  12. pPD1cR

    Plasmid
    #86970
    Purpose
    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, G108C in PCNA1
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86970-plasmid-map-sequence-id-173927

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  13. pPD2cX

    Plasmid
    #86968
    Purpose
    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…
    Promoter
    T7 promoter
    Available Since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    86968-plasmid-map-sequence-id-173869

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  15. pCAP03-act1p

    Plasmid
    #120233
    Purpose
    Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.
    Depositor
    Bradley Moore
    Article
    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-
    Insert
    promoter cassette containing act1p promoter and apramycin resistance gene
    Use
    Synthetic Biology
    Mutation
    aac(3)IV (apramycin resistance gene)
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    120233-plasmid-map-sequence-id-233753

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  17. pCas9GG

    Plasmid
    #131009
    Purpose
    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Bba_R0040 TetR
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131009-plasmid-map-sequence-id-257082

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  18. pCas9FnCpf1GG

    Plasmid
    #131013
    Purpose
    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131013-plasmid-map-sequence-id-257083

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  19. pPD3cC

    Plasmid
    #86972
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86972-plasmid-map-sequence-id-173929

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  23. pCAP03-sp44/p21

    Plasmid
    #120232
    Purpose
    Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.
    Depositor
    Bradley Moore
    Article
    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-
    Insert
    bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
    Use
    Synthetic Biology
    Mutation
    aac(3)IV (apramycin resistance gene)
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    120232-plasmid-map-sequence-id-233750

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  27. pPtGE34

    Plasmid
    #107932
    Purpose
    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Article
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107932-plasmid-map-sequence-id-242338

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  28. LeGO-iBSD-5LO-WT

    Plasmid
    #182741
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available Since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182741-plasmid-map-sequence-id-360423

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  30. pAAV-UbC-tdTomato

    Plasmid
    #62516
    Purpose
    Expresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures
    Depositor
    Jae Lee, Pantelis Tsoulfas
    Article
    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/
    Insert
    tdTomato
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    hUbC
    Available Since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    62516-plasmid-map-sequence-id-107809

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  33. LeGO-iBSD-5LO-d4

    Plasmid
    #182743
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform delta 4 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available Since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182743-plasmid-map-sequence-id-367810

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  34. LeGO-iBSD-5LO-d13

    Plasmid
    #182745
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform delta 13 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Available Since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182745-plasmid-map-sequence-id-367797

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  36. LeGO-iBSD-5LO-p12

    Plasmid
    #182744
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform p12) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform p12 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available Since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182744-plasmid-map-sequence-id-367805

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  37. LeGO-iBSD-5LO-E376Q

    Plasmid
    #182742
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (enzymatically inactive E376Q mutant) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase E376Q (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    enzymatically inactive 5-LO E376Q mutant, codon o…
    Promoter
    SFFV
    Available Since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182742-plasmid-map-sequence-id-367803

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  39. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Article
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107999-plasmid-map-sequence-id-217151

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  44. AiP14871: pAAV-AiE0779m_3xC2-minBG-RiboL1-jGCaMP8s-WPRE3-BGHpA (Alias: CN4871) (AAV1)

    Viral Prep
    #214865-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AiP14871: pAAV-AiE0779m_3xC2-minBG-RiboL1-jGCaMP8s-WPRE3-BGHpA (Alias: CN4871) (#214865). In addition to the viral particles, you will also receive purified AiP14871: pAAV-AiE0779m_3xC2-minBG-RiboL1-jGCaMP8s-WPRE3-BGHpA (Alias: CN4871) plasmid DNA. Neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s under the minBG promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only

  45. AiP15571: pAAV-AiE1426m-minBG-iCre(R297T)-BGHpA (Alias: CN5571) (AAV2)

    Viral Prep
    #230877-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from AiP15571: pAAV-AiE1426m-minBG-iCre(R297T)-BGHpA (Alias: CN5571) (#230877). In addition to the viral particles, you will also receive purified AiP15571: pAAV-AiE1426m-minBG-iCre(R297T)-BGHpA (Alias: CN5571) plasmid DNA. minBG-driven expression of iCre(R297T) in striatal Sst-Chodl interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Available Since
    Nov. 14, 2025
    Availability
    Academic Institutions and Nonprofits only

  46. AiP15646: pAAV-AiE1426m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5646) (AAV2)

    Viral Prep
    #230884-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from AiP15646: pAAV-AiE1426m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5646) (#230884). In addition to the viral particles, you will also receive purified AiP15646: pAAV-AiE1426m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5646) plasmid DNA. minBG-driven expression of SYFP2 in cortical and striatal Sst-Chodl neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Available Since
    Nov. 14, 2025
    Availability
    Academic Institutions and Nonprofits only

  47. AiP13738 - pAAV-AiE0743m_3xC2-minBG-tdTomato-WPRE3-BGHpA (Alias: CN3738) (AAV2)

    Viral Prep
    #224173-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from AiP13738 - pAAV-AiE0743m_3xC2-minBG-tdTomato-WPRE3-BGHpA (Alias: CN3738) (#224173). In addition to the viral particles, you will also receive purified AiP13738 - pAAV-AiE0743m_3xC2-minBG-tdTomato-WPRE3-BGHpA (Alias: CN3738) plasmid DNA. minBG-driven expression of tdTomato in striatal cholinergic neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Tags
    tdTomato
    Available Since
    Nov. 14, 2025
    Availability
    Academic Institutions and Nonprofits only

  48. AiP13780: pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780) (AAV2)

    Viral Prep
    #214848-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from AiP13780: pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780) (#214848). In addition to the viral particles, you will also receive purified AiP13780: pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780) plasmid DNA. Expression of iCre(R297T) in striatal cholinergic neurons under the minBG promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Available Since
    Nov. 14, 2025
    Availability
    Academic Institutions and Nonprofits only

  49. AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) (AAV PHP.eB)

    Viral Prep
    #199777-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) (#199777). In addition to the viral particles, you will also receive purified AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) plasmid DNA. Cre recombinase expression in striatal indirect pathway medium spiny neurons (D2-MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only

  50. AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) (AAV PHP.eB)

    Viral Prep
    #199776-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) (#199776). In addition to the viral particles, you will also receive purified AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) plasmid DNA. Cre recombinase expression in striatal direct pathway medium spiny neurons (D1-MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 201 - 250 of 18460 results