Addgene

We narrowed to 503 results for: abo.4

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BB2_L_FG_syn_BbsI

Plasmid

#89922

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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BB2_L_GH_syn_BbsI

Plasmid

#89923

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pKD237-3a-2

Plasmid

#90957

Purpose

Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_J23100, Bba_J23105, and PphsA

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pKD239-1g-2

Plasmid

#90959

Purpose

Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Tetrathionate response regulator
Superfolder GFP
mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_J23100, Bba_J23109, and PttrB185-269

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pKD184

Plasmid

#90953

Purpose

Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_J23114, PltetO-1, and PphsA

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pKD233.7-3

Plasmid

#90955

Purpose

Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Tetrathionate response regulator
Superfolder GFP
mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_J23114, PltetO-1, and PttrB185-269

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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BB1_O_34_AMA1_2.8

Plasmid

#90293

Purpose

BB1 with AMA1_2.8 sequence for transient plasmid propagation in A. niger between FS 34

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

AMA1_2.8

Use

Tags

Expression

Bacterial

Mutation

it is a truncated version of AMA1 (5.3 kb)

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST694_BB3_pTet-on_mttA_trpC_pcoxA:pyrGtrunc_AMA2.8

Plasmid

#90292

Purpose

BB3 for mttA integration with homologous flanks at pyrG locus, hygromycin resistance

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

mttA

Use

A niger

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBWB162

Plasmid

#140634

Purpose

pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-pgk-puro

Plasmid

#85747

Purpose

tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mutation

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBWB294

Plasmid

#140636

Purpose

pBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAS_gpyrG1

Plasmid

#90276

Purpose

(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg1)

Use

A. niger

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCAS_gpyrG2

Plasmid

#90277

Purpose

(also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg2)

Use

A. niger

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90285

Purpose

BB2 with split pyrG marker for homologous integration with linker AB (negative control)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Use

Tags

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90286

Purpose

BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Use

Tags

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90287

Purpose

BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Use

Tags

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90288

Purpose

BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Use

Tags

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pH-eCFP

Plasmid

#176555

Purpose

Expression of eCFP for auxotrophic selection in the absence of histidine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

eCFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pL-eCFP

Plasmid

#176556

Purpose

Expression of eCFP for auxotrophic selection in the absence of leucine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

eCFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pU-eCFP

Plasmid

#176557

Purpose

Expression of eCFP for auxotrophic selection in the absence of uracil

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

eCFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge