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Addgene
Showing: 81 - 100 of 537 results
  1. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid
    #227327
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Insert
    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid
    #227321
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Insert
    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. qTAG-N-Solo-sTagRFP-TUBA1B

    Plasmid
    #207765
    Purpose
    Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Insert
    TUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. qTAG-N-Blast-moxGFP-TUBA1B

    Plasmid
    #207766
    Purpose
    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Insert
    TUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. qTAG-N-Puro-moxGFP-TUBA1B

    Plasmid
    #207767
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Insert
    TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid
    #73220
    Purpose
    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Insert
    dCas9 from S. pyogenes
    Use
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PpsbA2
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA-GFP-EBA175-Flag(Y1426S)

    Plasmid
    #155035
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    Y1426S; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA-GFP-EBA175-Flag(P1424C)

    Plasmid
    #154978
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424C variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424C; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA-GFP-EBA175-Flag(P1424M)

    Plasmid
    #154979
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424M variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424M; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pcDNA-GFP-EBA175-Flag(P1424V)

    Plasmid
    #154988
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424V; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA-GFP-EBA175-Flag(P1424L)

    Plasmid
    #154989
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424L variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424L; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pcDNA-GFP-EBA175-Flag(P1424F)

    Plasmid
    #154990
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424F variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424F; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pcDNA-GFP-EBA175-Flag(P1424Y)

    Plasmid
    #154991
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424Y variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424Y; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. pcDNA-GFP-EBA175-Flag(P1424W)

    Plasmid
    #154992
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424W variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424W; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. pcDNA-GFP-EBA175-Flag(P1424T)

    Plasmid
    #154993
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424T variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424T; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. pcDNA-GFP-EBA175-Flag(P1424S)

    Plasmid
    #154994
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424S; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 81 - 100 of 537 results