We narrowed to 56,262 results for: plasmid

1. DTECT - Plasmid for Standard Curve

   Plasmid

   #139333

   Purpose

   This plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECT

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   DTECT ligation product
   

   Use

   Unspecified

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Non-targeting CRISPRi plasmid

   Plasmid

   #154891

   Purpose

   Human non-targeting CRISPRi gRNA

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

   Insert

   Nontargeting gRNA
   

   Use

   CRISPR and Lentiviral

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)

   Plasmid

   #127636

   Purpose

   Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

   Insert

   mTurquoiuse2-Tat, YPet-PCP
   

   Use

   Synthetic Biology

   Tags

   RNA binding peptide: PCP and RNA binding peptide:…

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LSAM domain expression plasmid

   Plasmid

   #240215

   Purpose

   Plasmid for the recombinant expression of the LSAM domain of human legumain in E. coli.

   Depositor

   Elfriede Dall

   Article

   Dahms et al STAR Protocols, 6, 3, 103991, 19 Septemb

   Insert

   LSAM (LGMN Human)
   

   Tags

   PC1pro

   Expression

   Bacterial

   Available Since

   July 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAHW/Joseph2-Multitagged Plasmid

   Plasmid

   #226725

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pAHW/Joseph2
   

   Use

   Drosophila s2 cell expression

   Tags

   HA, Myc, GFP, Flag, V5, 6xHis

   Expression

   Insect

   Promoter

   Act5C

   Available Since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pU6-hL1-5'_3.3kb plasmid

   Plasmid

   #226006

   Purpose

   Plasmid used to generate DNA-FISH probe targeting at human L1HS

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   Human L1 element 3.3kb sequences at the 5' end
   

   Use

   Synthetic Biology

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. eIF3k sgRNA-2 PX459 plasmid

   Plasmid

   #192256

   Purpose

   expressing sgRNA-2 targeting eIF3k locus closed to stop coden

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. eIF3k sgRNA-1 PX459 plasmid

   Plasmid

   #192255

   Purpose

   expressing sgRNA-1 targeting eIF3k locus closed to stop coden

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eIF3f sgRNA-1 PX459 plasmid

   Plasmid

   #192253

   Purpose

   expressing sgRNA-1 targeting eIF3f locus closed to stop coden

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. eIF3f sgRNA-2 PX459 plasmid

    Plasmid

    #192254

    Purpose

    expressing sgRNA-2 targeting eIF3f locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. eIF3b sgRNA-2 PX459 plasmid

    Plasmid

    #192250

    Purpose

    expressing sgRNA-2 targeting eIF3b locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. eIF3b sgRNA-1 PX459 plasmid

    Plasmid

    #192249

    Purpose

    expressing sgRNA-1 targeting eIF3b locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RPS4X sgRNA-2 lentiCRISPR v2 plasmid

    Plasmid

    #192232

    Purpose

    lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RPS4X sgRNA-1 lentiCRISPR v2 plasmid

    Plasmid

    #192231

    Purpose

    lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RPS15A sgRNA-2 lentiCRISPR v2 plasmid

    Plasmid

    #192228

    Purpose

    lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RPS15A sgRNA-1 lentiCRISPR v2 plasmid

    Plasmid

    #192227

    Purpose

    lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ppk301-T2A-QF2 HDR plasmid

    Plasmid

    #130667

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    

    Use

    Donor template

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJRH-1362 scFv entry plasmid

    Plasmid

    #201912

    Purpose

    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BcLOV4 fusion tools screening plasmid #1

    Plasmid

    #174511

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BcLOV4 fusion tools screening plasmid #2

    Plasmid

    #174512

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge