We narrowed to 10,165 results for: trac

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pSCIP-hTRAC

Plasmid

#154094

Purpose

Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA and BAIT targeting human TRAC locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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Topo_SpCas9.tracr_LbCas12a.DR

Plasmid

#155049

Purpose

TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)

Use

CRISPR

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAdTrack shALAS-1

Plasmid

#22748

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shALAS-1 (Alas1 Mouse)

Use

Adenoviral and RNAi

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pAdTrack-mC/EBPbeta

Plasmid

#49196

Purpose

adenoviral shuttle vector with mouse C/EBPbeta

Depositor

Jed Friedman

Insert

C/EBPbeta LAP fragment (Cebpb Mouse)

Use

Adenoviral

Promoter

CMV

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only
pAdTrack-mLIP

Plasmid

#49197

Purpose

adenoviral shuttle vector with mouse C/EBPbeta LIP fragment

Depositor

Jed Friedman

Insert

C/EBPbeta (LIP fragment) (Cebpb Mouse)

Use

Adenoviral

Mutation

only the LIP fragment

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pAdTrackCMV-PSM-Rb

Plasmid

#37107

Depositor

Joseph Nevins

Article

Angus et al Oncogene. 2012 Jan 16. doi: 10.1038/onc.

Insert

Rb (RB1 Human)

Use

Adenoviral

Expression

Mammalian

Mutation

aa 379-928 only; large pocket of human Rb with aa…

Promoter

CMV

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac1;2

Plasmid

#47765

Purpose

expresses AmTrac1;2 in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac1;2

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available Since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-LS

Plasmid

#47767

Purpose

expresses AmTrac-LS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-LS

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pDR-meptrac

Plasmid

#47768

Purpose

expresses MepTrac in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

meptrac

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-IS

Plasmid

#47766

Purpose

expresses AmTrac-IS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac1;2

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
hSyn-tractin

Plasmid

#187649

Purpose

Expresses F-tractin (from ITPKA)-mNeongreen under control of human synapsin promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

F-tractin

Use

AAV

Tags

mNeonGreen

Expression

Mammalian

Promoter

Synapsin

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p5'UTRAct2 (GB0193)

Plasmid

#68178

Purpose

Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

5'UTR AtAct2

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac-GS

Plasmid

#47769

Purpose

expresses AmTrac-GS in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac-GS

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
NYESO beta/alpha into TRAC HDRT Source (pTR 169)

Plasmid

#112021

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta/alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available Since

Sept. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

Plasmid

#85146

Purpose

lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mRuby3-P2A-mTurquoise-MLC

Use

Lentiviral

Tags

mRuby3, mTurquoise

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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[#GS5-RV] MitoTRACER-Donor

Plasmid

#233505

Purpose

Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Retroviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-UltraCas12a-6xNLS

Plasmid

#209023

Purpose

Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

UltraCas12a-6xNLS-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

M537R/F870L

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 F-tractin-EGFP

Plasmid

#58473

Purpose

Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

F-tractin (Itpka Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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