Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,991 results for: LEA

Showing: 2451 - 2500 of 15991 results

  1. C113m

    Plasmid
    #121012
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Cas9
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121012-plasmid-map-sequence-id-236320

    Enlarge

  2. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

    Enlarge

  3. 371-A

    Plasmid
    #97026
    Purpose
    yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97026-plasmid-map-sequence-id-182505

    Enlarge

  4. pTRIPZ (M)-HT-NLS

    Plasmid
    #82518
    Purpose
    Lentivirus vector. Expresses Halotag proteins with nuclear localization signal in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Nuclear localizaition sequence
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82518-plasmid-map-sequence-id-156794

    Enlarge

  5. mTagBFP-Nucleus-7

    Plasmid
    #55265
    Purpose
    Localization: Nuclear Localization, Excitation: 399, Emission: 456
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    mTagBFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55265-plasmid-map-sequence-id-475376

    Enlarge

  6. pcDNA3-TopBP1-mCer

    Plasmid
    #211414
    Purpose
    mammalian expression of human TopBP1 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    TopBP1 (TOPBP1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211414-plasmid-map-sequence-id-420477

    Enlarge

  7. pOpen-K12polLF

    Plasmid
    #165511
    Purpose
    DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.
    Depositor
    FreeGenes Project, Jennifer Molloy, Drew Endy
    Insert
    DNA Polymerase I, Large (Klenow) Fragment
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165511-plasmid-map-sequence-id-338596

    Enlarge

  8. pcDNA3.1-aASIC1

    Plasmid
    #32795
    Depositor
    Cecelia Canessa
    Insert
    ASIC1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pAIP4_3A7L

    Plasmid
    #234182
    Purpose
    Heterologous protein expression of glycine cleavage system H protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3A7L (GCV3 Escherichia coli K-12)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234182-plasmid-map-sequence-id-464147

    Enlarge

  10. pcDNA3-RecQL4-mCer

    Plasmid
    #211402
    Purpose
    mammalian expression of human RecQL4 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    RecQL4 (RECQL4 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211402-plasmid-map-sequence-id-420438

    Enlarge

  11. 371-U

    Plasmid
    #97036
    Purpose
    MYFQSNA-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    MYFQSNA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97036-plasmid-map-sequence-id-182502

    Enlarge

  12. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid
    #52421
    Purpose
    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry
    Depositor
    Bertrand Collet
    Insert
    Fusion NLS-EGFP-P2A-Cherry-CAAX
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    52421-plasmid-map-sequence-id-81252

    Enlarge

  13. EBFP2-Nucleus-7

    Plasmid
    #55249
    Purpose
    Localization: Nuclear Localization, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    EBFP2-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. mEmerald-Nucleus-7

    Plasmid
    #54206
    Purpose
    Localization: Nucleus, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    mEmerald-Nucleus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. U25m

    Plasmid
    #121027
    Purpose
    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RiboJ + UTR1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121027-plasmid-map-sequence-id-238073

    Enlarge

  18. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Richard Baker
    Inserts
    groEL
    groES
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228495-plasmid-map-sequence-id-454882

    Enlarge

  19. pCer-C3-Plk1

    Plasmid
    #68132
    Purpose
    Expression of Cerulean-tagged Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available Since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68132-plasmid-map-sequence-id-123472

    Enlarge

  20. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Promoter
    T7-lac0
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

    Enlarge

  21. pN_UBQ10/mApple

    Plasmid
    #140405
    Purpose
    mApple expression regulated by the A. thaliana UBQ10 promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)
    Depositor
    Mathias Pribil
    Insert
    mApple
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    AtUBQ10
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    140405-plasmid-map-sequence-id-292694

    Enlarge

  22. pHL1726

    Plasmid
    #53032
    Purpose
    PLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and hfqlead
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. pHL1790

    Plasmid
    #53038
    Purpose
    PLtetO-1:glgClead::mCherry, PLlacO-1:csrB
    Depositor
    Han Lim
    Inserts
    mCherry
    csrB
    Use
    Synthetic Biology
    Tags
    glgC leader
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Charles Gersbach
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69545-plasmid-map-sequence-id-125605

    Enlarge

  25. pExp-DsbC

    Plasmid
    #112564
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-DsbC and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112564-plasmid-map-sequence-id-217214

    Enlarge

  26. WT_S1-H1

    Plasmid
    #218392
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    218392-plasmid-map-sequence-id-436407

    Enlarge

  27. pcDNA3-BG4-NLS-mCer

    Plasmid
    #211448
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Tags
    NLS-mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211448-plasmid-map-sequence-id-419419

    Enlarge

  28. N1-LOC8R

    Plasmid
    #66896
    Purpose
    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.
    Depositor
    Georg Ramm
    Insert
    LOC8R
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66896-plasmid-map-sequence-id-114924

    Enlarge

  29. pExp-DsbA(SS)

    Plasmid
    #112563
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-DsbA(SS) and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112563-plasmid-map-sequence-id-217221

    Enlarge

  31. pHL1746

    Plasmid
    #53034
    Purpose
    PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ycdTlead
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. pAIP4_5GUQ

    Plasmid
    #234171
    Purpose
    Heterologous protein expression of ASCH domain-containing ribonuclease in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5GUQ
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234171-plasmid-map-sequence-id-464137

    Enlarge

  33. WT_H1-S1

    Plasmid
    #218400
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in first intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    218400-plasmid-map-sequence-id-442676

    Enlarge

  34. MUT_S1-N

    Plasmid
    #218398
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218398-plasmid-map-sequence-id-433922

    Enlarge

  35. MUT_N-H1

    Plasmid
    #218397
    Purpose
    3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218397-plasmid-map-sequence-id-433918

    Enlarge

  36. WT_S7-H1

    Plasmid
    #218393
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218393-plasmid-map-sequence-id-433906

    Enlarge

  37. pQE80l B-CC-B

    Plasmid
    #196479
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC
    Depositor
    Fei Sun
    Insert
    B-CC-B
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196479-plasmid-map-sequence-id-387244

    Enlarge

  38. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

    Enlarge

  39. pHL1747

    Plasmid
    #53035
    Purpose
    PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ydeHlead
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pHL1722

    Plasmid
    #53030
    Purpose
    PLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and glgC leader
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  41. pHL1745

    Plasmid
    #53033
    Purpose
    PLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and cstAlead
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. pHL1724

    Plasmid
    #53031
    Purpose
    PLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and pgaAleadFull
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. pHL1914

    Plasmid
    #53042
    Purpose
    PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Han Lim
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and hfqlead
    Expression
    Bacterial
    Promoter
    PconNoHindM2 and pLlacO-1
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. pET-Impact-T4gp43(exo-)

    Plasmid
    #228202
    Purpose
    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A
    Depositor
    Stephen Benkovic
    Insert
    43
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated aspartate 219 to alanine (D219A), elimina…
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228202-plasmid-map-sequence-id-453943

    Enlarge

  47. pCer-C3-Plk1-K82R

    Plasmid
    #68134
    Purpose
    Expression of Cerulean-tagged, kinase-dead Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    K82R kinase-dead version
    Promoter
    CMV
    Available Since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68134-plasmid-map-sequence-id-123466

    Enlarge

  49. MUT_S1-H1

    Plasmid
    #218394
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218394-plasmid-map-sequence-id-433909

    Enlarge

  50. pCer-C3-Plk1-T210D

    Plasmid
    #68133
    Purpose
    Expression of Cerulean-tagged, constitutively active Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    T210D constitutively active version
    Promoter
    CMV
    Available Since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68133-plasmid-map-sequence-id-123512

    Enlarge

Showing: 2451 - 2500 of 15991 results