Addgene

We narrowed to 4,534 results for: EED

Showing: 981 - 1000 of 4534 results

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pLT 651

Plasmid

#59998

Purpose

Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

Depositor

Lisa Timmons

Article

Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

Inserts

klp-16 (klp-16 Nematode)
him-8 (him-8 Nematode)

Use

Tags

Expression

Bacterial

Mutation

partial genomic

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)

Plasmid

#169079

Purpose

Human ATG3

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

ATG3 (ATG3 Human)

Use

Tags

6X Histidine Tag, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7 lac promoter

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pg-proPHD2-PHD2-EGFP

Plasmid

#87210

Purpose

PHD2-EGFP expressed under the control of the PHD2 promoter

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

PHD2 (EGLN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

H313N

Promoter

PHD2

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBabepuro3-p18 A61D

Plasmid

#24937

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p18 (CDKN2C Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

A61D

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-FL

Plasmid

#34597

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

Plasmid

#123194

Purpose

binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304-MARK3

Plasmid

#107235

Purpose

Lentiviral gene expression vector for phospho-MARK3 expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS

Plasmid

#158628

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway entry vector

Tags

3XFLAG

Expression

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-myc3-Mdm2 D367A

Plasmid

#52058

Purpose

expression of non-cleavable Mdm2 in mammalian cells

Depositor

Trudy Oliver, Tyler Jacks

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Mdm2 D367A (MDM2 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

D367A

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pLX304-MARK3_T211A

Plasmid

#107236

Purpose

Lentiviral gene expression vector for phospho-MARK3_T221A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1CAN

Plasmid

#178032

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

Promoter

T7LacI

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLT 652

Plasmid

#59997

Purpose

Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

Depositor

Lisa Timmons

Article

Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

Inserts

dhc-3 (dhc-3 Nematode)
him-8 (him-8 Nematode)

Use

Tags

Expression

Bacterial

Mutation

partial genomic

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-LRRK2 Armadillo 1-552

Plasmid

#186017

Purpose

2xHis-LRRK2 Armadillo 1-552

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-LRRK2 Armadillo 1-552 (LRRK2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG

Plasmid

#163719

Purpose

Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG

Use

Tags

Expression

Bacterial

Mutation

dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSCT-GAL93-NONO

Plasmid

#46325

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

NONO (NONO Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only
pBpuro - HA KLF4 FL

Plasmid

#34589

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO_HOXB13_#2

Plasmid

#70094

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLKO_HOXB13_#1

Plasmid

#70093

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
lenti-Cas9-VQR-Blast

Plasmid

#87155

Purpose

lentiviral expression of SpCas9-VQR (NGA PAM restricted)

Depositor

Daniel Bauer

Article

Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

Insert

SpCas9-VQR(D1135V,R1335Q,T1337R)

Use

Lentiviral

Tags

Expression

Mutation

D1135V,R1335Q,T1337R

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-321/+120

Plasmid

#35540

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

none

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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