We narrowed to 29,674 results for: REP

1. Dual opposing T7 promoters reporter plasmid

   Plasmid

   #156457

   Purpose

   A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Insert

   NeoR/KanR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-REPORT(PGK/CMV)-GtwyA

   Plasmid

   #208574

   Purpose

   LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   mnucTomato
   HSVtk
   Neomycin
   d2nucEGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK/CMV and minP

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-mScarlet-TEV-Strep-His

   Plasmid

   #210823

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mScarlet
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPPI4-Strep-SNAP-CTLA-4-His6

   Plasmid

   #223583

   Purpose

   Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   Tags

   His6 and Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-CMV-TetO2_3C-Twin-Strep

   Plasmid

   #113883

   Purpose

   Mammalian (inducible) protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-TWIN-STREP-FLAG-FLCN

   Plasmid

   #164981

   Purpose

   FLCN expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Use

   Tags

   2xSTREP FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-FLAG-2xSTREP-CCND3-Puro

   Plasmid

   #172619

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC446 - pAAV EF1a hPREP(S554A)

   Plasmid

   #59968

   Purpose

   An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S554A

   Promoter

   EF1a

   Available since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-Twin-Strep-TEV-DGKζ

   Plasmid

   #202531

   Purpose

   Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   DGKZ (DGKZ Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and chicken β-actin promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. M78 pIRESpuro-CBP-2xTEV-StrepTag

    Plasmid

    #17132

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Type

    Empty backbone

    Use

    Tags

    2xTEV, CBP, and Streptavidin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-SMS1-TEV-Twin-Strep

    Plasmid

    #202528

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS1 (SGMS1 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    CAG and chicken β-actin promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21-Streptavidin-Dead Aspartate Loop

    Plasmid

    #46368

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Dead Aspartate Loop
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pLV-REPORT (PGK) 5xGRE-NanoLuc

    Plasmid

    #207170

    Purpose

    GAl4 response element driven NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Roennfeldt et al Biomolecules. 2023 Oct 19;13(10):1545. d

    Inserts

    NanoLuciferase
    d2eGFP
    5x GRE
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mutation

    Promoter

    Gal4 response element minP and PGK

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

    Plasmid

    #172647

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pvalb-2A-Flpo Flp-in replacement vector

    Plasmid

    #61572

    Purpose

    Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    Pvalb-2A-Flpo
    

    Use

    Recombinase-mediated cassette exchange using flp …

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-mCherry-P2A-strepII-PPPCB

    Plasmid

    #67923

    Purpose

    Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-FLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172626

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 GIL-11 myc TEV TwinStrep

    Plasmid

    #199627

    Purpose

    Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

    Depositor

    Jurgen Scheller

    Article

    Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

    Insert

    GIL-11
    

    Use

    Tags

    TwinStrep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GB1-PRD(703-800)-Strep

    Plasmid

    #141344

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

    Insert

    GB1-His6-TEV-ALIX(703-800)-Strep
    

    Use

    Tags

    GB1-His6-TEV and Strep-tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSETB-S28 histone 3 phosphorylation reporter

    Plasmid

    #32788

    Depositor

    Alice Ting

    Article

    Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

    Insert

    CFP-14-3-3tau-H3 peptide-YFP
    

    Use

    Tags

    CFP and YFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only