We narrowed to 172,713 results for: Gene

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pLVX-TetOneZeo-ORF18(WT)-3xFLAG

Plasmid

#129746

Purpose

Dox inducible expression of KSHV ORF18-3xFLAG in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE3Gs and Tet inducible promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

Plasmid

#110572

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD-stRBS-aacC1

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pFS478

Plasmid

#89066

Purpose

KanMX Z3EV promoter cassette

Depositor

Nick Rhind

Article

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Insert

Z3EV promoter cassette

Use

Yeast integration vector

Mutation

yeast Gal1 promoter modified with multiple Zif268…

Promoter

Z3EV promoter

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pAGM37443

Plasmid

#153218

Purpose

Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-3xT7-nSREBP1a

Plasmid

#171030

Purpose

Expresses 3xT7-tagged nuclear form of SREBP1a in mammalian cells

Depositor

Kai Ge

Article

Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

Insert

nSREBP1a (Srebf1 Mouse)

Use

Retroviral

Tags

3xT7

Expression

Mammalian

Mutation

amino acids 1- 449

Promoter

5' LTR

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBHM2406

Plasmid

#173442

Purpose

pRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR template

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

mNeonGreen-CBP-2XFLAG-HYGB

Tags

CBP-2xFLAG

Expression

Bacterial and Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC-ARS-CRISPR-v2

Plasmid

#99863

Purpose

Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMM113

Plasmid

#127210

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, AtSTM, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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MBD2b pcDNA3.1

Plasmid

#78142

Purpose

Mammalian expression of MBD2b

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD2b (MBD2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pKAd5f11p-EPG

Plasmid

#122697

Purpose

To generate E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and an EF1a promoter-controlled transgene.

Depositor

Zhuo-Zhuang Lu

Article

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Insert

GFP

Use

Adenoviral

Promoter

EF1a

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-G12-CheRiff-T2A-mCherry

Plasmid

#170275

Purpose

CheRiff-T2A-mCherry construct expression in mammlian cells. pAAV vector. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

CheRiff-T2A-mCherry

Use

AAV

Promoter

11xUAS with TATA-box minimal promoter

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-16

Plasmid

#160716

Purpose

T-nos Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

nopaline synthase terminator (T-nos) bordered by BsaI restriction sites

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-ZEAX

Plasmid

#53274

Purpose

Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli

Depositor

Francis X Cunningham Jr

Article

Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52

Insert

crtE, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Mutation

Deleted idi (ORF6) and crtX genes

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CamKIIa-ChR2-EGFP

Plasmid

#114216

Purpose

Expresses channelrhodopsin-EGFP fusion in CamKIIa+ neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Channelrhodopsin 2

Use

AAV and Mouse Targeting

Tags

EGFP

Expression

Mammalian

Promoter

CamKIIa 1.3 kb

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBI-p53 wt/EGFP

Plasmid

#16543

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p53 (TP53 Human)

Expression

Mammalian

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pVAX1-BMP7

Plasmid

#137910

Purpose

Constitutive mammalian overexpression vector for human bone morphogenetic protein 7 cDNA

Depositor

Georg Feichtinger

Article

Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

Insert

BMP7 (BMP7 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_T

Plasmid

#147556

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7 (SMG7 Human)

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G and one non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHS-393

Plasmid

#195473

Purpose

Lentiviral Integratable Inducible super-IL2 cytokine + synZiFTR10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF10 BS - ybTATA - IL12] - pSFFV - ZF10 - NS3 - p65

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD28 SNIPR

Plasmid

#188358

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD28-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBP-J23100

Plasmid

#72963

Purpose

Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

J23100 promoter

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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