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Showing: 10021 - 10040 of 65398 results

  6. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid
    #154072
    Purpose
    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.
    Inserts
    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    Tags
    GST, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    154072-plasmid-map-sequence-id-308806

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  10. pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v5 (B.1.617.3)

    Plasmid
    #212540
    Purpose
    Encodes SARS-CoV-2 spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D614G,P681R,D950N (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T19R,G142D,E156-F157 deletion,R158G,N450R,E484Q,D…
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212540-plasmid-map-sequence-id-431560

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  11. pTwist-SARS-CoV-2 Spike-deltaC18 SAK007134 VOC-501Y.V2 (B.1.351v6)

    Plasmid
    #212393
    Purpose
    Encodes SARS-CoV-2 spike A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N501Y,D614G,A701V (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    A27S,D80A,D215G,L242-L244 deletion,K417N,E484K,N5…
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212393-plasmid-map-sequence-id-431632

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  12. pVRC8400-SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D-AVI

    Plasmid
    #176332
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176332-plasmid-map-sequence-id-347141

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  13. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

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Showing: 10021 - 10040 of 65398 results