We narrowed to 22,641 results for: his
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Plasmid#173017PurposeImproved green-red FRET based cAMP indicator.DepositorInsertGR-ICUE2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag7SS-DogTag
Plasmid#171775PurposeExpresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formationDepositorInsertHaloTag7SS-DogTag
TagsHis6ExpressionBacterialMutationDogTag inserted in HaloTag7 between residues D139…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB601NL3F10H
Plasmid#141287PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)DepositorTypeEmpty backboneUseGateway Cloning and LuciferaseTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti TMEM30A
Plasmid#171789Purposetransfer plasmid for lentiviral vector production expressing TMEM30ADepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC-AncCAR_A
Plasmid#168286PurposeExpresses AncCAR_A in E. coli from a T7 promoterDepositorInsertAncCAR_A
UseSynthetic BiologyTags6*HisExpressionBacterialPromoterT7Available SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-TBP6.9F34MF37MF77M
Plasmid#168268PurposeExpresses human TBP6.9 with the F34M/F37M/F77M triple mutantDepositorInsertTBP6.9
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F34M, F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Q-IL6-9
Plasmid#166552PurposescFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptideDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, Sortag, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)
Plasmid#164193PurposeFreeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)DepositorInsertsCamPARI2
Human α Tubulin
UseAAVTags6 His tag and NESExpressionMammalianMutationF391W-L398VPromoterSynAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS D614 soluble Spike
Plasmid#164652PurposeExpresses SARS-CoV-2 soluble Spike proteinDepositorAvailable SinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3-NLS
Plasmid#138218PurposeNuclear-targeted FRET-based cAMP indicator.DepositorInsertICUE3-NLS
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Integrin-β6 Headpiece I270C
Plasmid#159638PurposeContains integrin beta 6 residues 1–474 with I270C mutationDepositorInsertIntegrin beta 6 headpiece I270C
Tags6x HisExpressionMammalianMutationI270CAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
53 pLIB_MBP
Plasmid#160301PurposeN-term 3C cleavable MBP tag for insect cell expressionDepositorTypeEmpty backboneTags6xHis and MBPExpressionInsectPromoterpolyhydrinAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR(T/A)
Plasmid#140052PurposeNegative control mutant for ABKAR biosensor.DepositorInsertABKAR(T/A)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceNov. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb719-HO
Plasmid#141200PurposeExpresses mRhubarb719 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb719
heme oxygenase
Tags6x-HisExpressionBacterialMutationR134H, Y198S, T202Y, V203I, W309R, Q310A compared…PromoterT7Available SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-H1.4
Plasmid#157799PurposeExpression of mEGFP and human linker histone H1.4 fusion proteinDepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK187
Plasmid#156423PurposeInsect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein
TagssfGFP-HisExpressionInsectPromoterPolyhedrinAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAK463
Plasmid#140701PurposeNBαMs-IgKDepositorInsertNBαMs-IgK
ExpressionBacterialAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.SUZ12
Plasmid#125172PurposeExpresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorAvailable SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.JARID2
Plasmid#125166PurposeExpresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only