We narrowed to 29,674 results for: REP

1. Pvalb-2A-Dre Flp-in replacement vector

   Plasmid

   #61571

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-2xFLAG-2xSTREP-CCND3-Puro

   Plasmid

   #172622

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter

   Plasmid

   #172596

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-R914W (PDGFRA Synthetic, Human)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-R914W

   Promoter

   CMV

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC-Z-SpCas9-gRNA-repeat-polyT-11

   Plasmid

   #197781

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   gRNA repeat for U6 sgRNA assembly
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-2xFLAG-2xSTREP-CCND2-Puro

   Plasmid

   #172628

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GB1-PRD(800-868)-strep

   Plasmid

   #180023

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)
   

   Use

   Tags

   GB1-His6-TEV and Strep tag (WSHPQFEK)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. human KANK1 Ankyrin Repeats pET151

   Plasmid

   #164053

   Purpose

   Expresses human KANK1 Ank domain in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc

   Plasmid

   #161587

   Purpose

   expression of Myc-tagged mouse Lrrk2 without GTPase domain

   Depositor

   Maik Hintze

   Insert

   Lrrk2 (Lrrk2 Mouse)
   

   Use

   Tags

   Myc tag and StrepII

   Expression

   Mammalian

   Mutation

   deletion: AA1023-1879

   Promoter

   CMV

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc

   Plasmid

   #161586

   Purpose

   expression of Myc-tagged mouse Lrrk2 without kinase domain

   Depositor

   Maik Hintze

   Insert

   Lrrk2 (Lrrk2 Mouse)
   

   Use

   Tags

   Myc tag and StrepII

   Expression

   Mammalian

   Mutation

   deletion: AA1871-2540

   Promoter

   CMV

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-REPORT(EF1a no GFP) mnuctdTomato

    Plasmid

    #172342

    Purpose

    mnuctdTomato

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ABCA1 promoter luciferase reporter (P1 fragment)

    Plasmid

    #86443

    Purpose

    Human ABCA1 promoter luciferase reporter (P1 fragment)

    Depositor

    David Mu

    Article

    Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

    Insert

    Human ABCA1 (ABCA1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid

    #158576

    Purpose

    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    

    Use

    Tags

    V5 and HA on the endogenous KCC2 protein product

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FL MALT1 Catalytic Mutant in pET29b-Strep

    Plasmid

    #48969

    Purpose

    Expresses Full length catalytic mutant MALT1 in e.coli

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 isoform A (MALT1 Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Catalytic Mutant C464A

    Promoter

    T7

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

14. ABCA1 promoter luciferase reporter (P2 fragment)

    Plasmid

    #86444

    Purpose

    Human ABCA1 promoter luciferase reporter (P2 fragment)

    Depositor

    David Mu

    Article

    Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

    Insert

    Human ABCA1 (ABCA1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. ABCA1 promoter luciferase reporter (P3 fragment)

    Plasmid

    #86445

    Purpose

    Human ABCA1 promoter luciferase reporter (P3 fragment)

    Depositor

    David Mu

    Article

    Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

    Insert

    Human ABCA1 (ABCA1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.2 mir-1-1 reporter hsa-let-7a-1

    Plasmid

    #46670

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-let-7a-1 (MIRLET7A1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

    Plasmid

    #139770

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal TwinStrep tag
    

    Use

    Tags

    3C - TwinStrep tag

    Expression

    Mutation

    Promoter

    PH

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL0376 (His-MBP-StrepII-TniQ)

    Plasmid

    #130641

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite-StrepII on TniQ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-mcherry-WIPI2d-cs-TEV-STREP

    Plasmid

    #178912

    Purpose

    Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d
    

    Use

    Tags

    C term strep and N term mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-FLAG-2xSTREP-CCND1-Puro

    Plasmid

    #172643

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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