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Plasmid#134306Purposemitochondria-targeted inverse-response citrate sensor variant with medium binding affinityDepositorInsertCitroff1 S245A
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUB-GFP
Plasmid#11155PurposeMammalian expression vector for expression of GFP (Ubiquitin C promoter)DepositorUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEXPqcxip-hSURF4-FLAG
Plasmid#159139PurposeHu SURF4 -flag mammalian expression Gateway vector.DepositorInsertsurfeit 4 (SURF4 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMV (cytomegalovirus) enhancer and the MSV (mouse…Available sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP3A4
Plasmid#67104PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP3A4 (CYP3A4 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tubulin-hfYFP
Plasmid#186515PurposeLocalization of hyperfolder YFP fluorescent protein to tubulinDepositorInsertHyperfolder YFP
UseTagsTubulinExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-6xNLS-E174R/S542R (pRG232)
Plasmid#149723PurposeLentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSens-4OmeNB
Plasmid#216231PurposeExpresses GFP in response to 4'-O-MethylnorbelladineDepositorInsertsRamR-4NB2
sfGFP
UseSynthetic BiologyTagsExpressionMutationK64T, L67M, C135D, S138GPromoterJ23103 and PramrAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZR060_CROPseq-sgCtrl-CRISPRi
Plasmid#180269PurposeLentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRiDepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP2D6
Plasmid#67090PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP2D6 (CYP2D6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWUR703
Plasmid#53082Purposeinducible TtAgoDM (D478A,D546A) expressionDepositorInsertTtAgo
UseTagsStrep-II tagExpressionBacterialMutationD478A D546APromoterT7Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJK2020
Plasmid#171932Purposeexpress MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operonDepositorInsertPmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
UseTagssuperfolder GFP, mCherryExpressionWormMutationPromoter-Available sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZR069_CROPseq-sgPTPRC-CRISPRi
Plasmid#180271PurposeLentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRiDepositorInsertPTPRC (CD45) sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSicoR
Plasmid#11579PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B-GFP
Plasmid#116869PurposeNuclear localized GFPDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNL4-3 envFS eGFP Δvpu/env
Plasmid#101345PurposeDeletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RREDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta vpu/EnvPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
puc19-pCas13a
Plasmid#118963PurposeIntermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.DepositorInsertCas13a
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNTI661 pRPR1(TetO)-sgRNA
Plasmid#139475PurposeVector for tet-regulated sgRNA expression in yeastDepositorInsertsingle guide RNA scaffold
UseCRISPRTagsExpressionYeastMutationPromoterpRPR1(TetO)Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hfYFP
Plasmid#186523PurposeHyperfolder YFP fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertHyperfolder YFP
UseTagsExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only