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pET28a-His-hPKM2

Plasmid

#42515

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 (PKM Human)

Tags

His

Expression

Bacterial

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only
Cathepsin S S21C/C25A

Plasmid

#84695

Purpose

for expression of mutant Cathepsin S in Leishmania tarantolae

Depositor

Hans Brandstetter

Article

Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

Insert

cathepsin S (CTSS Human)

Use

Expression in leishmania tarantolae

Tags

his-tag

Mutation

S21C, C25A

Promoter

unknown

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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XE124 XDsh delta DEP-GFP-CS2+

Plasmid

#16785

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta-DEP (dvl2.L Frog)

Use

Xenopus expression

Tags

GFP

Mutation

contains Dsh amino acids 1-433 and 503-736 (delet…

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-TRAF4

Plasmid

#58316

Purpose

FLAG-tagged mouse TRAF4

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5 MEKK4alpha KD

Plasmid

#12188

Depositor

Gary Johnson

Article

Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

Insert

MEKK4 (Map3k4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Kinase dead: K1361M

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Hes5

Plasmid

#61536

Purpose

Expresses Hes5 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes5 (Hes5 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pHM123DEnv

Plasmid

#137668

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3_altPKA-Cα-mEGFP

Plasmid

#114132

Purpose

Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

18747 (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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MO70-mJP-4

Plasmid

#79598

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

Insert

Junctophilin-4 (Jph4 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pLL5.0 hCortKD EGFP

Plasmid

#187267

Purpose

Lentiviral expression of shRNA targeting Cortactin

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

shRNA for Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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ATG9A-2STrep

Plasmid

#210359

Purpose

Expresses ATG9A fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

ATG9A (ATG9A Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Tollip M240A,F241A

Plasmid

#178051

Purpose

GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Methionine 240 to Alanine and changed Phe…

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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L1dRSLE

Plasmid

#13266

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Expression

Bacterial

Mutation

RSLE region removed

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
Ad-EFNA1

Plasmid

#102724

Purpose

Expression of EphrinA1

Depositor

Jin Chen

Article

Brantley-Sieders et al Cancer Res. 2006 Nov 1;66(21):10315-24.

Insert

EFNA1 (EFNA1 Human)

Use

Adenoviral

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMIR-CDH1 wtUTR

Plasmid

#25038

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 3'UTR (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pBS ColI alpha2

Plasmid

#19207

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Alpha 2 type I collagen (COL1A2 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pgRGFP

Plasmid

#82695

Purpose

Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

PGK, U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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LacI-11/109-pHG165c

Plasmid

#90059

Purpose

Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI-11/109

Expression

Bacterial

Mutation

109

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

Plasmid

#128404

Purpose

Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Exotoxin A (toxA P. aeruginosa)

Tags

6xHis tag and DsbA signal sequence for periplasmi…

Expression

Bacterial

Mutation

242DNNNS246 and 384DQNRT388 engineered glycosylat…

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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HMGA2-FLAG

Plasmid

#25409

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

high mobility group AT-Hook 2 (Hmga2 Mouse)

Use

Retroviral; Chip

Tags

FLAG

Expression

Mammalian

Mutation

None

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only